| 囊尾蚴胶原蛋白的提取与分析 |
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| 引用本文： | 王凯慧,李雅杰. 囊尾蚴胶原蛋白的提取与分析[J]. 寄生虫与医学昆虫学报, 1999, 6(2): 94-98 |
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| 作者姓名： | 王凯慧 李雅杰 |
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| 作者单位： | 哈尔滨医科大学寄生虫学教研室 |
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| 摘 要： | 作者首次从猪囊尾蚴中提取胶原蛋白，用紫外分光仪、ＳＤＳ－ＰＡＧＥ、透射电镜和ＥＬＩＳＡ等方法对猪囊尾蚴胶原蛋白进行了初步分析。表明：（１）猪囊尾蚴中含有一定量的胶原蛋白；（２）胶原蛋白主要以Ⅰ型为主，分子量在８０ｋＤａ～１３０ｋＤａ之间；（３）通过酶联免疫吸附实验，检测其抗原性。对４２例脑囊虫病患者血清ＩｇＧ抗体的检测，阳性率达８８．１％，而对３０例正常人检测结果均为阴性。说明囊尾蚴胶原蛋白对人具有很强的免疫性，其抗原决定簇可刺激机体产生特异性ＩｇＧ抗体。因此猪囊尾蚴胶原蛋白是具有很强的抗原性的一组蛋白质，有望成为囊虫病新的免疫诊断用抗原。
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| 关 键 词： | 囊尾蚴 胶原蛋白 囊虫病 IgG |
EXTRACTION AND ANALYSIS OF CYSTICERCUS COLLAGEN |
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| Wang KaihuiLi Yajie Xu ZhijieBian Xiaoyan. EXTRACTION AND ANALYSIS OF CYSTICERCUS COLLAGEN[J]. Acta Parasitologica et Medica Entomologica Sinica, 1999, 6(2): 94-98 |
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| Authors: | Wang KaihuiLi Yajie Xu ZhijieBian Xiaoyan |
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| Abstract: | Cysticercus collagen is fistly extracted from cysticercusand preliminarily analysed with ultraviolet spectrophotometer,SDSPAGE,transmission electron microscope(TEM) and ELISA.The resules revealed:There are a certain amount of collagen in cysticercus;The major collagen which the molecular weight are 80130 kDa are belong to tape ;42 patients with cysticercosis and 30 cases of normal persons are tested with ELISA to determine the specility IgG in the serum.The positive rate is 881%.So cysticercus collagen possess stronger immunity and contains a group of protein possessing intensive antigenicity.It is definitely worth whilewith respect to the antigens of cysticercosis immunodiagnosis. |
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| Keywords: | CysticercusCollagenCysticercosisIgG |
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