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Induction of specific anti-actin antibodies and their measurement by ELISA technique. |
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| Authors: | D Charlemagne K Schwartz S Bernard A Paraf |
| Affiliation: | 1. ERA No. 802 C.N.R.S., Station de Recherches de Virologie et d''Immunologie de l''I.N.R.A., Laboratoire d''Immunologie Cellulaire de l''E.P.H.E., 78850 Thiverval-Grignon, France;2. Station de Recherches de Virologie et d''Immunologie, I.N.R.A., 78850 Thiverval-Grignon, France;3. I.N.S.E.R.M. (U. 127), Unité de Recherche sur le Métabolisme du Coeur et des Vaisseaux, Hôpital Lariboisière, 41, Boulevard de la Chapelle, 75010 Paris, France |
| Abstract: | Using the ELISA technique we have been able to quantify antibodies directed against actin and to follow the kinetics of antibody production. Specific anti-actin antisera have been raised in rabbits by immunization with chemically modified white muscle rabbit actin. Two or three dinitrophenyl groups linked per actin molecule were sufficient to break natural tolerance, while linkage of three phosphorylcholine groups to actin was not. |
| Keywords: | A buffer 0.5 M sodium carbonate, pH 9.5 BSA bovine serum albumin DNP dinitrophenol ELISA enzyme-linked immunosorbent assay FCA Freund's complete adjuvant G-actin monomeric actin HMM heavy meromyosin OD optical density OVA ovalbumin PC phosphorylcholine SDS sodium dodecyl sulphate TM tropomyosin TN troponin |
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