表皮生长因子及其受体在胃癌中的表达

目的：观察表皮生长因子（ＥＧＦ）及其受体（ＥＧＦＲ）在胃癌中的表达，加深对胃癌发生分子基础的了解。方法：采用免疫组织化学方法（ＡＢＣ法），分析探讨两者在５２例原发性胃癌组织中的变化。结果：ＥＧＦ、ＥＧＦＲ在胃癌中的阳性表达率分别为４０４％（２１／５２）和４８１％（２５／５２），两者同时阳性表达率为３６５％（１９／５２）；ＥＧＦ在中、晚期胃癌，淋巴结转移胃癌，低分化胃癌中的免疫阳性反应率为４７６％（２０／４２）；５３１％（１７／３２）；５２３％（１８／３４），分别高于其在早期（１０％，１／１０）、淋巴结阴性（２０％，４／２０）、高分化胃癌（１６７％，３／８）中的表达，Ｐ＜００５；ＥＧＦＲ表达与临床进展无明显相关性，Ｐ＞００５，但其在淋巴结转移（５９４％，１９／３２）、低分化胃癌（６１８％，２１／３４）中的表达率分别高于其在淋巴结阴性（３０％，６／２０）、高分化胃癌（２２２％，３／１８）中的表达，Ｐ＜００５。结论：ＥＧＦ、ＥＧＦＲ在胃癌转移过程中起一定作用，并可能是胃癌恶性程度的影响因素。

Expression of EGF and EGFR in Gastric Cancer

Objective: To investigate alteration EGF and EGFR in gastric cancer. Methods: Immunohistochemistry staining (ABC tech) was carried out. Results: Immunohistochemical positive reaction for EGF and EGFR was 40.4%(21/52),48.1%(25/52) respectively,in 52 gastric cancer analyzed, 19 case had a concurrent positive immunostaining(36.5%) of EGF and EGFR; expression of EGF was 47.6%(20/42),53.1%(17/32),52.3%(18/34) respectively in advaced;lymph-node metastasis and poorly differentiation gastric cancer, which was significantly higher than that in the early,no lymph-node metastasis and differentiation ones (10%,1/10;20%,4/20;16.7%,3/18), respectively,P<005; EGFR expression did not correlate with clinical stage, P>005; but its positive-staining rate had significant difference between lymph-node involved gastric cancer(59.4%,19/32) and non-lymphnode involved ones(30%,6/20) or between poorly differentiation(61.8%,21/34) and differntiation gastric cancer(22.2%,3/18), P<005.Conclusion: The results indicated that EGF and EGFR play a role in the metastasis of gastric cancer and that those have an effect on degree of malignance.