| 超氧阴离子自由基对NIH3T3细胞的转化作用 |
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| 引用本文： | 房克忠 俞志宏. 超氧阴离子自由基对NIH3T3细胞的转化作用[J]. 基础医学与临床, 1996, 16(2): 49-52 |
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| 作者姓名： | 房克忠 俞志宏 |
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| 作者单位： | 中国协和医科大学基础医学院，中国医学科学院基础医学研究所 |
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| 摘 要： | 首次选取ＮＩＨ３Ｔ３细胞，对比检测外源性Ｏ＾－２和致癌，促癌剂争生转化的能力。结果表明，Ｏ＾－２单独作用组较正常对照组和启动剂量Ｂ（ａ）Ｐ单独作用组之转化率提高了近３倍；当Ｏ＾－２与启动剂量Ｂ（ａ）Ｐ协同作用时，转化率上述两组分别提高约１１倍和１０倍；而用ＳＯＤ和ＣＡＴ清除胞外Ｏ＾－２时，转化率下降８２％。
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| 关 键 词： | 超氧阴离子 NIH3T3 细胞转化 致癌 促癌 自由基 |
Transformation of NIH3T3 Cells by Superoxide Anion |
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| Fang Kezhong, Yu Zhihong, Qiu Qi, Jiang Jianghong, Wu Yuande. Transformation of NIH3T3 Cells by Superoxide Anion[J]. Basic Medical Sciences and Clinics, 1996, 16(2): 49-52 |
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| Authors: | Fang Kezhong Yu Zhihong Qiu Qi Jiang Jianghong Wu Yuande |
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| Abstract: | he transformation frequency(TF)of N IH3T3 cells was examined to study the effect ofextracellular superoxide anion(O2)on cell transformation,The TF in cell cultures treated withO2 alone was 3 times greater than that in control group(A) or that in cultures received initiatingdose of B(a)P alone(B).In cultures treated with TPA in combination with B(a)P,the TF wasabout 20 and l8 times greater than that in group A and B respectively.When and B(a) Pacted cooperately,TF was nearly 11 and 10 times greater than that in group A and B respective-ly.Superoxide dismutase(SOD)in combination with catalase(CAT),as scavengers of activeoxygen,reduced the TF by 82%,These results show that can act as a complete carcinogenespecially as a promoter. |
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| Keywords: | Superoxide anion NIH3T3 Transformation Initiation Promotion |
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