UPLC同时测定不同产地川芎中8种活性成分的含量

目的建立同时测定不同产地川芎中咖啡酸、香草醛、阿魏酸、洋川芎内酯I、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞含量的UPLC方法。方法采用ACQUITY UPLC,HSS T3色谱柱(2.1 mm×100 mm,1.8μm),流动相为甲醇-0.03%磷酸水溶液,梯度洗脱,PDA检测器,针对不同组分,选择其最大吸收波长作为检测波长。结果咖啡酸、香草醛、阿魏酸、洋川芎内酯I、洋川芎内酯H、丁基苯酞、藁本内酯和丁烯基苯酞分别在0.082 8～3.312μg.mL 1,0.047 85～1.914μg.mL 1,4.420～176.8μg.mL 1,2.035～81.4μg.mL 1,0.470 4～18.82μg.mL 1,1.044～41.75μg.mL 1,13.55～542.0μg.mL 1,1.785～71.40μg.mL 1内呈良好的线性关系,平均回收率分别为101.0%,99.1%,98.9%,103.6%,96.3%,102.8%,98.1%,98.0%。结论该方法简便、快捷、重复性好,可用于川芎药材及其制剂的质量控制。

Simultaneous Determination of Eight Active Components in Chuanxiong Rhizoma from Different Areas by UPLC

OBJECTIVE To develop a UPLC method for determination of caffeic acid, vanillin, ferulic acid, senkyunolide I, senkyunolide H, butylphthalide, ligustilide and butylidenephthalide in Chuanxiong Rhizoma from different areas. METHODS The analysis was carried out on an HSS T3 （2.1 mmx100 mm, 1.8μm） column. The mobile phase was composed of 0.03% phosphoric acid aqueous and methanol with gradient elution. The detection wavelengths were set at three different wavelengths according to different components. RESULTS The standard curves of eight active components showed a good linearity in 0.0828-3-312μg·mL^-1,0.04785-1·914μg.mL^-1,4.420-176·8μg.mL^-1,2.035-81·4μg.mL^-1,O.4704-18·82μg.mL^-1,1.044-41·75μg.mL^-1,13.55-542·0μg＇mL^-1,1.785-71·40μg·mL^-1The average recoveries were 101.0%, 99.1%, 98.9%, 103.6%, 96.3%, 102.8%, 98.1%, 98.0%, respectively. CONCLUSION This method is simple, quick, reproducible and can be used to control the quality of Chuanxiong Rhizoma and its preparations.