川芎嗪、山莨菪碱、蝮蛇抗栓酶及卡托普利对糖尿病大鼠肾脏的保护作用

目的　探讨川芎嗪、山莨菪碱、蝮蛇抗栓酶及卡托普利对实验大鼠糖尿病肾病 (DN)的预防作用。方法　SD大鼠 6 0只 ,分为正常对照组、糖尿病组及各糖尿病治疗组。以四氧嘧啶腹腔内注射制成糖尿病模型。各治疗组分别给予上述药物治疗 ,每周 5次 ,共 14周。取肾脏行光镜和电镜检查 ,并进行图像分析 ,同时测定蛋白糖基化产物 (GP)。结果　与糖尿病组比较 ,各治疗组肾组织中水平明显降低 (P <0 .0 1) ;除蝮蛇抗栓酶组外 ,其他治疗组大鼠肾小球截面积亦明显缩小 (P <0 .0 5 ) ;糖尿病组大鼠肾小球基底膜显著不均匀增厚 ,而各治疗组病变较轻 ,尤以卡托普利组疗效最为明显。结论　改善微循环、血液流变学及血流动力学治疗可减慢实验大鼠DN的进展。

Protective effect of tetramethylpyrazine, anisodamine, ahalysan tinfarctase and captopril on kidney of experimental diabetic rats

Objective To re search the prophylactic effect of ligustrazine, anisodamine, ahalysan tinfarctase and captopril for diabetic nephropathy (DN) of rats. Methods Sixty SD rats were divided randomly into six groups: normal control group (NC), diabetes control group (DC), and diabetes treated group 1-4 (DT1-4). The diabetes model was established by intraperitoneal injection of alloxan. The ligustrezine, anisodamine, ahalysan tinfarctase and captopril were given respectively for each DT rats, 5 times a week for 14 weeks. The kidneys were processed for morphological examination with light and electron microscopy, and the glucosylation of protein (GP) in renal tissue was assayed. Results Compared with DC, the levels of GP in renal tissues in each DT was decreased significantly (P<0.01), and the glomerular plana area in each DT was also reduced (P<0.05) except for the ahalysan tinfarctase treated group. Electron microscopic findings indicated that the glomerular basal membrane in DC rats was uneven thick significantly, and these lesion were less severe in each DT rats, especially in captopril-treated group. Conclusion With improving microcirculation, blood rheology and hemodynamics could slow the progress of the DN in experimental diabetes rats.