骨炎定对鸡膝关节骨性关节炎氧自由基代谢的影响

目的研究骨炎定对鸡实验性膝关节炎氧自由基代谢的影响，探讨骨炎定防治骨性关节炎的机理。方法选取鸡40只，随机分成正常组、模型组、骨炎定低剂量组、骨炎定高剂量组和芬必得组。骨炎定低剂量组、骨炎定高剂量组分别灌胃5g／kg、10g／kg药物，芬必得组灌胃0．15g／kg药物。分别测定各组血清超氧化物歧化酶活性和丙二醛含量。结果模型组鸡血清超氧化物歧化酶活性下降，与正常组比较有显著性差异（P〈0．05）。骨炎定低剂量组、高剂量组能提高骨性关节炎鸡血清SOD活性，与模型组、正常组比较均有极显著性差异（P〈0．01），芬必得组和正常组无显著性差异（P〉0．05）。模型组鸡血清含量MDA上升，与正常组比较有极显著性差异（P〈0．01）。骨炎定低剂量组、高剂量组能降低骨性关节炎鸡血清MDA含量，与模型组比较有显著性差异（P〈0．01～0．05），芬必得组与正常组无显著性差异（P〉0．05）。结论骨炎定可通过提高实验性膝关节炎鸡血清SOD活性，清除体内过剩自由基，降低血清MDA含量，防治氧自由基对患病关节的损伤，达到治疗骨性关节炎的目的。

Effect of Guyanding Decoction on the Metabolism of Oxygen- derived Free Radicals in Chicken Knee Osteoarthritis

Obstract: Objective To study the effect of Guyanding Decoction(GYD) on the metabolism of oxygen-derived free radicals in the chicken model of knee osteoarthritis and to explore its therapeutic mechanism.Method Forty chickens were randomized into 5 groups: normal group,model group,low-dose GYD group,high-dose GYD group and Fenbid group.Low-dose group was administered 5 g/kg GYD and high-dose group with 10 g/kg GYD by gastric gavage.Fenbid group was given Fenbid 0.15 g/kg.The SOD activity and the MAD content in serum were detected in each group.Result The activity of SOD in model group was significantly lower than that in normal group(P < 0.05).The GYD in low and high doses could increase the activity of SOD,the difference being significant as compared with the normal group and model group(P < 0.01),while the SOD activity in Fenbid group did not differ from the normal group(P > 0.05).For the content of MDA,model group had a higher level than normal group(P < 0.01),and GYD in low and high doses could decrease MDA content(P < 0.05 or P < 0.01 as compared with the model group),while MDA content in Fenbid group did not differ from the normal group(P > 0.05).Conclusion The decoction of GYD exerts curative effect on experimental osteoarthritis by improving the activity of SOD,eliminating excessive oxygen-derived free radicals,decreasing the content of MDA,and preventing osteoarthritis patients from the injury of oxygen-derived free radicals.