| 人IL_2活性测定及其影响因素的探讨 |
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| 引用本文： | 王鲁男,李淑华,潘兴瑜.人IL_2活性测定及其影响因素的探讨[J].辽宁医学院学报,1990(3). |
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| 作者姓名： | 王鲁男 李淑华 潘兴瑜 |
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| 作者单位： | 锦州医学院微生物学教研室，锦州医学院微生物学教研室，锦州医学院微生物学教研室 |
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| 摘 要： | 作者建立了小鼠胸腺细胞MTT比色分析法并检测了33份正常人的IL_2活性。探讨了影响人IL_2活性测定的因素并与~3H-TdR掺入法进行比较。结果表明,该法简便,稳定,可行,与~3H-TdR掺入法比较二者有较好的一致性,认为本法可用于临床IL_2样本的检测。
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| 关 键 词： | 白细胞介素2 胸腺细胞 MTT比色分析 |
A STUDY OF THE ASSAY OF HUMAN INTERLEUKINE_2 (IL_2) ACTIVITY AND INFLUENCED FACTORS |
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| Wang Lunan et al.A STUDY OF THE ASSAY OF HUMAN INTERLEUKINE_2 (IL_2) ACTIVITY AND INFLUENCED FACTORS[J].Journal of Liaoning Medical University (LNMU) Bimonthly,1990(3). |
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| Authors: | Wang Lunan |
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| Abstract: | The MTT Colorimetric assay was established by using thymocytes of mice as target cells and the IL_2 activity was measured in 33 normal individuals. The factors affecting the IL_2 activity were studied and Compared with ~3H-TdR in corporation assay. The resucr has shown that this assay is convenient, reproducible and practicable. By Comparing MTT with ~3H-TdR incorporation assay, there was good correlation between them. This method is considered available for use of the assay of Clinical IL_2 Samples. |
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| Keywords: | Interleukin 2 (IL_2) Thymocyte MTT colorimetric assay |
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