| 壳聚糖纳米载体提高抗乙肝免疫核糖核酸免疫活性的研究 |
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| 引用本文： | 张红菱,李媛媛,李晓波,孙纳,徐顺清. 壳聚糖纳米载体提高抗乙肝免疫核糖核酸免疫活性的研究[J]. 中国医院药学杂志, 2005, 25(8): 693-695 |
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| 作者姓名： | 张红菱 李媛媛 李晓波 孙纳 徐顺清 |
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| 作者单位： | 1. 武汉工业学院医学院,湖北,武汉,430023;华中科技大学同济医学院,湖北,武汉,430030
2. 华中科技大学同济医学院,湖北,武汉,430030 |
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| 摘 要： | 目的:通过制备抗乙肝免疫核糖核酸(iRNA)的壳聚糖纳米载体,提高其免疫活性.方法:复凝聚方法制备壳聚糖-免疫核糖核酸纳米粒,扫描电镜、原子力显微镜、Zeta电位/粒度分析仪观察测定壳聚糖-免疫核糖核酸纳米粒形态、粒径和表面电位;核糖核酸酶(RNase)保护试验观察壳聚糖对包裹的iRNA的保护作用;白细胞黏附抑制(LAI)试验测定壳聚糖-免疫核糖核酸纳米粒免疫活性.结果:壳聚糖与iRNA形成表面带正电荷的纳米粒,iRNA未被RNase的降解,白细胞黏附抑制率增高.结论:壳聚糖纳米载体提高了抗乙肝免疫核糖核酸的免疫活性.
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| 关 键 词： | 壳聚糖 免疫核糖核酸 纳米粒 白细胞黏附抑制 |
| 文章编号： | 1001-5213(2005)08-0693-03 |
| 收稿时间： | 2004-12-15 |
| 修稿时间： | 2004-12-15 |
The study on the anti-hepatitis B iRNA immune enhancing activities of chitosan nanoparticles |
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| ZHANG Hong-ling,LI Yuan-yuan,LI Xiao-bo,Sun Na,XU Shun-qing. The study on the anti-hepatitis B iRNA immune enhancing activities of chitosan nanoparticles[J]. Chinese Journal of Hospital Pharmacy, 2005, 25(8): 693-695 |
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| Authors: | ZHANG Hong-ling LI Yuan-yuan LI Xiao-bo Sun Na XU Shun-qing |
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| Affiliation: | 1. Medical college of Wuhan Polytechnic University, Hubei Wuhan 430023, China;2. Tongji Medical College Huazhong University of Science and Technology, Hubei Wuhan, 430030, China |
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| Abstract: | OBJECTIVE The aims of this study is to increase the immune activities of anti-hepatitis B immune RNA(iRNA) by prepare chitosan nanoparticles. METHODS Chitosan-iRNA nanoparticles were prepared by complex coacervation method. Measurements of particle shape,size and zeta potential of nanoparticles were performed by scan electromicroscopy,atom force microscopy,photon correlation spectroscopy and lazer Doppler anemometry respectively. By using leukocytes adherence inhibition(LAI) test the immune activities of the nanoparticles were evaluated. RESULTS The chitosan and iRNA formed the nanoparticles with positive electrical charge;iRNA was protected against RNase. And the nanoparticles exhibited an ability to inhibit leukocytes adherence.CONCLUSION The immune activities of iRNA in the chitosan-iRNA complex are enhanced. |
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| Keywords: | chitosan immune RNA nanoparticles leukocytes adherence inhibition |
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