| 分泌抗活化小鼠T细胞抗原单克隆抗体杂交瘤细胞株的建立 |
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| 引用本文： | 李宪昌,何球藻. 分泌抗活化小鼠T细胞抗原单克隆抗体杂交瘤细胞株的建立[J]. 复旦学报(医学版), 1991, 0(3) |
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| 作者姓名： | 李宪昌 何球藻 |
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| 作者单位： | 上海医科大学基础医学部免疫学教研室(李宪昌)，上海医科大学基础医学部免疫学教研室(何球藻) |
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| 基金项目： | 国家教委博士点基金资助课题(880092) |
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| 摘 要： | 用Con A活化的小鼠脾脏细胞免疫同品系动物(BALB/c鼠),取免疫鼠的脾细胞与小鼠骨髓瘤细胞(SP2/0)融合,选择了一株有分泌抗活化小鼠T细胞抗原抗体的杂交瘤细胞,经有限稀释法连续4次克隆化。采用ELISA法分析,初步证实该杂交瘤细胞所分泌的单克隆抗体(McAb)与Con A 活化的小鼠脾细胞的结合反应明显高于与未经活化的其他细胞(胸腺细胞、肠系膜淋巴结细胞、脾细胞)的反应。用琼脂双扩散法证明该MeAb属于小鼠IgG类。
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| 关 键 词： | 活化的小鼠T细胞抗原 同品系间免疫 单克隆抗体 |
ESTABLISHMENT OF A HYBRIDOMA CELL LINE SECRETING MONOCLONAL ANTIBODIES AGAINST ACTIVATED MOUSE T LYMPHOCYTE ANTIGEN |
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| Li Xianohang,He Qiuzao. ESTABLISHMENT OF A HYBRIDOMA CELL LINE SECRETING MONOCLONAL ANTIBODIES AGAINST ACTIVATED MOUSE T LYMPHOCYTE ANTIGEN[J]. Fudan University Journal of Medical Sciences, 1991, 0(3) |
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| Authors: | Li Xianohang He Qiuzao |
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| Abstract: | We succeeded in selecting a hybridoma cell line secreting antibodies against activated mouse T lymphocyte antigen by means of fusing between mouse myeloma cells (SP2/0) with spleen cells from BALB/o mice immunized with Oon A aotivaed syngenio spleen cells.The hybridoma cell line wes recloned 4 times successively by a limiting dilution method.Preliminary analysis by ELISA showed that the binding reaction of this monoclonal antibody to activated mouse spleen cells was significantly stronger than with other non-stimulated cells (thymocytes,mesentery lymph node cells and spleen cells).The monoclonal antibody is proved by double immunodiffusion method to be of the mouse IgG class. |
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| Keywords: | activated mouse T lymphocyte antigen syngeneie immunization monoclonal antibody |
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