Further studies of the turnover of dog antithrombin III. Study of 131I-labelled antithrombin protease complexes |
| |
Authors: | B Leonard R Bies T Carlson E B Reeve |
| |
Affiliation: | 2. Department of Medicine, University of Colorado Health Sciences Center, Denver, CO, USA 80262;1. Department of Biochemistry, University of New Mexico, Albuquerque, NM, USA 87131 |
| |
Abstract: | Fresh plasma containing 131I-antithrombin III (*I-AT) was coagulated and incubated at 37 degrees C for 2 hr. A "complex peak," separated on heparin-agarose contained AT and *I-AT antigen but no heparin cofactor activity. Crossed immunoelectrophoresis showed only AT complexes. SDS PAGE showed 80% of the *I-AT in a major band (approximately 80,000 daltons), 15% in a minor band (approximately 100,000 daltons) and the rest in trace bands (approximately 60,000 and/or 115,000 daltons). Ammonia treatment of the complex peak released alpha-thrombin. After i.v. injection 80% of the complexed *I-AT, chiefly as the major band, left the plasma with t 1/2 approximately 15 min and was almost immediately catabolized to low molecular weight breakdown products. A major catabolic site was the liver. A simple kinetic model describes the findings approximately. |
| |
Keywords: | Anti-thrombin III heparin crossed immunoelectrophoresis α-thrombin |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|