Division of Basic Medical Science, School of Medicine, Meiyuan Branch of Southern Yangtze University;Division of Basic Medical Science, School of Medicine, Meiyuan Branch of Southern Yangtze University;Division of Basic Medical Science, School of Medicine, Meiyuan Branch of Southern Yangtze University;Division of Basic Medical Science, School of Medicine, Meiyuan Branch of Southern Yangtze University;Division of Basic Medical Science, School of Medicine, Meiyuan Branch of Southern Yangtze University;Division of Basic Medical Science, School of Medicine, Meiyuan Branch of Southern Yangtze University
Abstract:
BackgroundThe high concentration of glutamate release is the main cause for neuronal cell death. The relationship between glutamate level and apoptosis during ischemia/reperfusion injury is still unclear.ObjectiveTo observe the neuronal apoptosis at 24 and 72 hours following cerebral ischemia/reperfusion in rats, and analyze the possible influencing factors.DesignA randomized controlled animal experiment.SettingSchool of Medicine, Southern Yangtze University.MaterialsTotally 30 male adult Sprague Dawley (SD) rats of clean grade, weighing 240–290 g, were obtained from Shanghai Experimental Animal Center, Chinese Academy of Sciences. The rats were randomly divided into sham-operated group (n=10) and model group (n=20). Each group was observed at 24 and 72 hours after ischemia/reperfusion, 5 rats at each time point in the sham-operated group, whereas 12 at 24 hours and 8 at 72 hours in the model group. Kits for determining apoptosis and Bcl-2 were bought from Wuhan Boster Biological Technology, Co., Ltd.; Kit for calcineurin from Nanjing Jiancheng Bioengineering Institute.MethodsThe experiment was carried out in the Functional Scientific Research Room of Southern Yangtze University from June to October in 2006.