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Genesis of a new spleen focus-forming virus: possible role of MCF viruses |
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| Authors: | D Barbieri-Weill D Mathieu-Mahul F Gay J Robert-Lezenes F Moreau-Gachelin F Wendling C Lacombe N Casadevall P Tambourin C J Larsen |
| Affiliation: | 1. G.R. N° 8 CNRS Institut Gustave-Roussy B.P. N° 8, 9.4800 Villejuif, France;2. Lab. de Pharmacologie Expérimentale, Centre Hayem, Hôpital Saint-Louis, 75010 Paris, France;3. U.248 Faculté de Médecine Saint-Louis-Lariboisière, 75010 Paris, France;4. U.22 Institut Curie, 91405 Orsay, France |
| Abstract: | A Friend mink cell focus-inducing (Fr-MCF) virus isolated from a Friend tumor cell line was able to induce acute erythroleukemia associated with polycythemia when injected as a Friend murine ecotropic leukemia virus (F-MuLV) pseudotype into adult Swiss and ICFW mice. One virus isolate recovered from leukemic cells and designated as FV-F3 presented the following properties: (i) persistence of the same leukemogenic power when propagated in vivo and in vitro; (ii) in vivo spleen focus-forming (SFFV) capacity; (iii) presence of erythropoietin (EPO)-independent CFU-E in leukemic animals; (iv) expression of a 32 RNA specifically recognized by a SFFV probe, in FV-F3 infected cells; and (v) expression in FV-F3-infected cell of polypeptides in the range of gp52 SFFV. Peptide analysis of these products revealed close similarities with the parental MCF virus. These data suggest that a SFFV genome arose by genetic recombinational events involving MCF virus. |
| Keywords: | Author to whom requests for reprints should be addressed. |
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