银杏内酯B对急性胆管炎大鼠细菌移位的作用

目的 观察银杏内酯B对重症胆管炎大鼠细菌移位的作用。方法 取Wistar大鼠，分3组：假手术组、感染组、治疗组，各12只。术前用荧光素标记大肠杆菌给鼠灌胃后，在感染组、治疗组制成急性胆管炎模型。假手术组只游离胆管。术后30min感染组腹腔内注射二甲亚砜，治疗组注射银杏内酯B。6h后活杀取肠系膜淋巴结和肝制成为10％组织匀浆在荧光显微镜下细菌计数。同时光镜下观察小肠和肝病理形态变化。另选取12只大鼠，用4％镧行肠系膜上静脉灌注随后用戊二醛灌注内固定，取该段小肠处理后在电镜观察镧在肠粘膜屏障的通透性。结果 治疗组各细菌移位数较感染组均显著减少(P＜0．01)。肠粘膜和肝病理损害也明显轻(P＜0．05)。电镜下，治疗组镧在肠粘膜通透性下降。结论 银杏内酯B可以减少急性胆管炎时肠道细菌向肠系膜淋巴结和远位器官的移位，并对肠粘膜屏障和肝等组织器官有保护作用。

Effects of ginkgolide B on bacteria translocation after severe cholangitis in rats

Objective To investigate the effects of ginkgolide B on bacteria translocation in rats with severe cholangitis. Methods Thirty-six Wistar rats were equally divided into the sham-operative group, infected group and therapeutic group. Before disposing, the rats were orally administered with E coli marked with fluorescence. The rats in the infected and therapeutic groups were used to establish the model of cholangitis and then the bile duct was isolated in both groups. In the sham-operated group, only separation of the bole duct was performed. Thirty minutes after the operation, rats in the infected group were intraperitoneally injected with dimethyl sulfoxide and those in the therapeutic group with ginkgolide B. Six hours later, the rats were killed and their mesenteric lymph nodes and liver were made into a 10% homogenate. Bacteria were counted under the fluorescent microscope. Meanwhile, the pathological changes of the small intestine and liver were observed. Another 12 rats were infused with 4% La through the mesenteric superior vein and glutaral internal fixation was conducted to observe the permeability of the barrier of intestinal mucosa. Results The bacteria translocation was significantly decreased and the pathological injury of the small intestine and liver was markedly milder in the therapeutic group as compared with the infected group (P<0.01, P<0.05). The permeability of La through the intestinal mucosa descended in the therapeutic group. Conclusions Ginkgolide B can reduce the bacteria translocation and protect the barrier of the intestinal mucosa and liver.