林可霉素生物合成基因lmbH的回转化

以ＰＩＪ７０２或ＰＩＪ４８６为载体构建了３个含有ｌｍｂＨ基因的重组质粒ｐＥＳＳ７０１、ｐＥＳＳ７０４和ｐＥＳＳ７０８分子，分别回转化林可霉素产生菌Ｂ４８，并随机挑选了６００个回转化克隆进行摇瓶初筛，以抑菌圈直长作为稀理林可霉素产生量的标准。

Backtransformation of lincomycin biosynthesis gene lmbH

Using pIJ702 or pIJ486 as vector, three recombinant plasmids pESS701, pESS704 and pESS708 which all contained lmbH gene were constructed, then they were backtransformed into S.lincolnensis B48 and 600 transformants were screened, respectively. Using the antibacterial inhibition zone to evaluate the lincomycin productivity level, the data indicated that at least one high producing strain was obtained from each backtransformation of the three recombinant plasmids and named as No.6, No.81 and No.244, respectively, when compared with the industrial parent strain. The relevant increasing rates of the lincomycin productivity level were 71.9%, 110% and 53.6%, respectively.