同一疫区汉坦病毒、恙虫病东方体共有宿主和媒介的分子流行病学研究

目的 了解汉坦病毒(HV)和恙虫病东方体(OT)在宿主和媒介体内的共生和自然感染情况.方法 采用现场流行病学调查,捕鼠166只、采螨3829只,用组织细胞培养和聚合酶链反应(PCR)技术从宿主和媒介体内分离和检测HV和OT病原体,以及HV-RNA、OT-DNA.结果 其中,捕获的105只黑线姬鼠中HV阳性3只,OT阳性2只,HV、OT同时阳性2只;41只褐家鼠中HV阳性2只,OT阳性1只,HV、OT同时阳性1只.从15份双重感染HV和OT阳性鼠体螨中分离到HV病原体2株,OT 2株,同时分离到HV和OT有1份.结论 HV和OT可同时自然感染并共生于宿主(鼠类)体内,而HV和OT能否同时感染媒介(恙螨)尚待证实.研究结果对HV和OT的流行和预防有重要的理论意义.

Molecular epidemiological study on the host and role of the Hantavirns and Orientia tsntsugamushi in the same epidemic area

Objective To investigate whether Hantavirus (HV) and Orientia tsutsugamushi (OT)can naturally infect and coexist in their host and role.Methods By field epidemiological study,Leptotrombidium scutellare(3829) was collected and separated from mice(166) in epidemic areas.The cells of mites separated from their host and role were cultured.PCR was used to detect HV-RNA and OT-DNA in the cell culture.Results In 105 Apodemus agrarius,3 HV-RNA positive,20T-DNA positive and 2 coinfection with HV and OT were deteeted;in 41 Brown rattus,2 HV-RNA positive,10T-DNA positive and 1 co-infection with HV and OT were detected.From 15 mites co-infected with HV and OT,2 strains of HV pathogen,2 strains of OT pathogen were separated and 1 HV and OT pathogen in the same mite were separate.Conclusion The study demonstrates that co-infection of HV and OT did simultaneously exist in wild Leptotrombidium scutellare,This theory has some significance to the epidemic and precaution of HV and OT.