| 人酸性成纤维细胞生长因子支持原代培养下丘脑神经元的存活和突起生长 |
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| 引用本文： | 赵桂玲,丁爱石,邵宁生,范明,刘毅,张双喜,魏文,王会信,王福庄. 人酸性成纤维细胞生长因子支持原代培养下丘脑神经元的存活和突起生长[J]. 军事医学科学院院刊, 1993, 0(3) |
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| 作者姓名： | 赵桂玲 丁爱石 邵宁生 范明 刘毅 张双喜 魏文 王会信 王福庄 |
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| 作者单位： | 山东医科大学生物学教研室(赵桂玲)，军事医学科学院基础医学研究所(丁爱石，邵宁生，范明，张双喜，魏文，王会信)，军事医学科学院仪器中心(刘毅)，军事医学科学院基础医学研究所(王福庄) |
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| 摘 要： | 初步研究了人酸性成纤维细胞生长因子(aFGF)对无血清培养条件下新生大鼠下丘脑神经元的突起生长和促生存作用。结果可见,在此培养物中加入纯化的aFGF后,神经元的存活数增加,神经突起生长,数目增多,aFGF的这种作用在一定范围内呈剂量效应关系。实验还表明,aFGF可增加神经元胞体直径和体积,流式细胞分析亦表明神经元蛋白总量提高。本结果提示,纯化的aFGF对在神经内分泌中占重要地位的下丘脑也有神经营养作用。其作用机制尚有待进一步研究。
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| 关 键 词： | 酸性成纤维细胞生长因子 下丘脑神经元 细胞培养 图像分析 |
Survival and neurite growth of hypothalamic neuron cultured under support of human acidic fibroblast growth factor |
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| Zhao Guiling,Ding Aishi,Shao Ningsheng,Fan Ming,Liu Yi,Zhang Shuangxi,Wei Wen,Wang Huixin,Wang Fuzhuang Institute of Basic Medical Sciences,Academy of Military Medical Sciences. Survival and neurite growth of hypothalamic neuron cultured under support of human acidic fibroblast growth factor[J]. Bulletin of the Academy of Military Medical Sciences, 1993, 0(3) |
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| Authors: | Zhao Guiling Ding Aishi Shao Ningsheng Fan Ming Liu Yi Zhang Shuangxi Wei Wen Wang Huixin Wang Fuzhuang Institute of Basic Medical Sciences Academy of Military Medical Sciences |
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| Affiliation: | Zhao Guiling,Ding Aishi,Shao Ningsheng,Fan Ming,Liu Yi,Zhang Shuangxi,Wei Wen,Wang Huixin,Wang Fuzhuang Institute of Basic Medical Sciences,Academy of Military Medical Sciences Department of Biology. Shandong Medical University Instrumental Analysis Centre. Academy of Military Medical Sciences |
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| Abstract: | The effects of human acidic fibroblast growth factor (aFGF) on the survival andneurite growth of hypothalamic neuron were observed in the cell culture with serum free medium.The results showed an increase both in the number of surviving cells and in the outgrowth ofneurites. In the presence of aFGF, the diameter and volume of neuron as well as the content ofprotein in the cells were markedly increased on image analysis and FACS. All effects were closelycorrelated with the dose. The results suggest that aFGF may play a neurotropic role in culturedhypothalamic neuron. |
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| Keywords: | acidic fibroblast growth factor hypothalamic neuron cell culture image analysis |
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