小鼠对表达狂犬病毒3aG株糖蛋白的重组复制缺陷型腺病毒的免疫应答

目的研究表达狂犬病毒3aG株糖蛋白(GP)的重组复制缺陷型腺病毒Ad/GP＇及Ad/GP免疫小鼠后所产生的特异性体液免疫应答，体外脾细胞增殖反应，及免疫小鼠对狂犬病毒致死性颅内攻击的保护力。方法1×10

Immune response of mice to replication-defective recombinant adenovirus containing glycoprotein gene of rabies virus 3aG strain

OBJECTIVE: To evaluate the immune response, of mice immunized with E1, E3 deleted replicative deficient recombinant adenovirus which can express glycoprotein of Chinese 3aG rabies virus. METHODS: Rapid fluorescent focus inhibition test (RFFIT) was used to determine the titer of neutralizing antibodies; specific antigen induced spleen lymphocyte proliferation was determined by in vitro 3H-TdR in-corporation assay, and lethal intracerebral challenge with CVS rabies virus was used to evaluate the protective effectiveness of the recombinant virus as candidate vaccine. RESULTS: Mice immunized by 1 x 10(7) recombinant virus developed high levels of rabies virus neutralizing antibodies (VNA). The spleen lymphocyte of the immunized mice also demonstrated remarkable increased proliferation after stimulation with inactivated rabies virus; 70%-90% of the mice immunized by the recombinant virus were protected from lethal intracerebral challenge with CVS rabies virus. CONCLUSION: E1, E3 deleted replication defective recombinant adenovirus containing 3aG glycoprotein gene may be developed as effective rabies vaccine to control rabies in China.