Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F |
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Authors: | Osmanağaoğlu O Beyatli Y Gündüz U |
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Affiliation: | Animal Science Department, University of Wyoming, Laramie 82071, USA. ozlemos@quark.fev.gazi.edu.tr |
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Abstract: | Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F. |
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