膜修饰脂质体对缺氧心肌细胞的保护作用

目的考察亲脂性化合物3{4[2羟基(1甲基乙胺基)丙氧基]苯基}丙酸十六醇酯(PAC)膜修饰脂质体对缺氧心肌细胞的保护作用。方法将普通脂质体(Plain L)、PAC膜修饰脂质体(PAC L)分别于常氧和缺氧条件下与乳鼠心肌细胞共同培养1、22、4 h,荧光法测定正常与缺氧心肌细胞对脂质体的摄取,以不加脂质体的细胞为对照,台盼兰染色法测定心肌细胞存活率。结果在常氧状态下1、2、24 h心肌细胞对PAC L的摄取较Plain L分别高约3.97、3.87、4.27倍,而在缺氧状态下心肌细胞对PAC-L的摄取较Plain L分别高约4.26、6.24、29.51倍。当细胞连续缺氧24 h时,对照组、Plain L组、PAC L组的心肌细胞存活率分别为7.8%、29.7%、95.4%。结论与Plain L相比,PAC L对缺氧状态的心肌细胞具有较强的亲和性,脂质体的加入能够明显降低缺氧对心肌细胞的损伤,PAC L对缺氧心肌细胞具有较强的保护作用,效果明显优于Plain L,且随着缺氧时间的延长这种优势越显著。

Protective effects of surface-modified liposome on the hypoxia cardiomyocytes

Objective To investigate the protective effects of 3-｛4-[2hydroxyl-(1-methyl ethylamino) propoxy]phenyl｝propionic acid cetylester(PAC) surface-modified liposome on the hypoxia cardiomyocytes.Methods The Plain-L and PAC-L were incubated with cardiomyocytes under the normoxic or hypoxia condition for 1 h,2 h,24 h.The uptakes of Plain-L and PAC-L by cardiomyocytes were measured by fluorescence spectrophotometer.Compared with the control group without liposome,the cardiomyocytes viability of Plain-L group and PAC-L group were evaluated by trypan blue exclusion test.Results Cardiomyocyte uptake of PAC-L under the normoxic condition were 3.97-fold,3.87-fold,and 4.27-fold higher than that of plain-L at 1 h,2 h and 24 h;The uptake of PAC-L under the hypoxia condition were 4.26-fold,6.24-fold,and 29.51-fold higher than that of plain-L.When anoxia persists 24 h,the cardiomyocyte viability of control group,Plain-L group and PAC-L group were 7.8%,29.7%,95.4%,respectively.Conclusions The affinity of PAC-L to hypoxia cardiomyocytes is higher than that of Plain-L.The protective effect of PAC-L against cardiomyocyte damnification induced by hypoxia is better than that of Plain-L.