原发性高血压患者WNK-SPAK激酶通路的表达及饮食干预的影响

目的：通过研究原发性高血压患者WNK1激酶（ with no lysine kinase 1）-STE20相关脯氨酸/丙氨酸丰富激酶（ Ste20-related proline/alanine-rich kinase,SPAK）信号通路的表达水平,以及低钠高钾饮食干预后表达水平的变化,探讨其在原发性高血压中的致病作用。方法：75名未经治疗的高血压患者经低钠高钾饮食干预1个月,通过Realtime PCR对干预前后外周血中的WNK1和SPAK的mRNA表达水平进行检测,并观察受试者血压、血尿生化指标变化。结果：高血压患者组外周血中WNK1和SPAK mRNA水平的表达较健康志愿者组明显升高,低钠高钾饮食干预后信号通路的表达则较基线期明显下降。统计学分析表明WNK1激酶的表达水平与血压之间存在相关性。结论：WNK1-SPAK激酶通路的表达异常可能参与原发性高血压的发病,饮食结构的变化可通过改变WNK1-SPAK-NCC信号通路的表达而调节血压。

The Expression of WNK-SPAK Kinase in Essential Hypertension Patients

Objective：To explore the pathogenic role of the WNK1（with no lysine kinase 1） -SPAK（Ste20-related pro-line/alanine-rich kinase） kinase signaling pathway in essential hypertension. We will investigate the expression of these kinases in essential hypertension patients before and after low sodium /high potassium diet transition. Methods：We detected the WNK1 and SPAK mRNA expression levels in the peripheral blood of 75 untreated hypertensive volunteers by Realtime PCR. And then we ob-served the changes in WNK1-SPAK cascade after low sodium and high potassium diet intervention for a month. Blood pressure,blood and urinary electrolytes were measured. Results：WNK1 and SPAK mRNA abundance in peripheral blood in hypertensive patients were increased significantly compared with healthy volunteers. The abundance of these kinases was significantly decreased after consuming low sodium /high potassium diet. Statistical analysis showed blood pressure was correlated with WNK1 kinase abundance. Conclusion：The expression of WNK1 and SPAK kinases pathway may be involved in the pathogenesis of essential hypertension. Low sodium high potassium diet may regulate blood pressure through inactivating WNK1-SPAK-NCC signaling pathway.