芡实对糖尿病肾病大鼠肾组织Urotensin Ⅱ及胶原表达的影响

目的：观察芡实对糖尿病肾病大鼠肾组织中UrotensinⅡ及胶原表达的影响。方法：雄性SD大鼠60只，随机选取10只大鼠为正常对照组（N组），其余50只采用一次性腹腔注射链脲佐菌素（STZ，45mg／kg）诱导DN模型，造模成功后随机分为糖尿病肾病组（DN）、芡实小剂量组（EL，1.5g·kg^-1·d^-1）、中剂量组（EM，3.0g·kg^-1·d^-1）、大剂量组（EH，6.0g·kg^-1·d^-1）及氯沙坦钾组（LP，30nag·kg^-1·d^-1），每组10只，均采用灌胃给药，N组和DN组给予等量蒸馏水。各组大鼠连续给药12周后，检测大鼠生化指标；HE、Masson染色观察肾脏病理变化；采用免疫组化、western—blot法测定Uro-tensinⅡ及胶原Ⅰ、Ⅲ在肾组织中表达的变化。结果：（1）与N组相比，12周末DN组大鼠肾小球肥大，系膜基质增生，间质胶原沉积．血清BUN、Scr、24h尿蛋白定量明显增高（P＜0.05），肾组织UrotensinⅡ及胶原Ⅰ、Ⅲ表达增加（P＜0.05）。（2）与DN组相比，EM、EH组大鼠病理改变减轻，BUN、Scr、24h尿蛋白定量显著下降（P＜0.05），肾组织UrotensinⅡ及胶原Ⅰ、Ⅲ表达明显降低（P＜0.05）。结论：芡实可通过抑制UrotensinⅡ及胶原Ⅰ、Ⅲ在肾闻质的过度表达，进而改善肾功能，延缓糖尿病肾病大鼠肾间质纤维化的进程，从而发挥肾脏保护作用。

Effect of Euryale Ferox on Expression of Urotensin Ⅱ and Collagen Ⅰ, IlI in Renal Tissues of Diabetic Nephropathy Rats

Objective ：To observe the effect of euryale ferox on expression of Urotensin lI and collagen in diabetic nephropathy rats. Methods：Sixty male Sprague -Dawley（SD） rats weighing 200 -220 g were used. Randomly selected 10 rats in control group （group N）, the remaining 50 making DN models injected streptozotocin into abdominal cavity（ STZ, 45 mg/kg）, were randomly divided into DN group （ group DN）, low - dose Euryale ferox group （ group EL, 1.5 g ·kg^-1·d^-1 ）, medium - dose Euryale ferox group（ group EM, 3.0 g· kg^-1 . d^-1）, high- dose Euryale ferox group（ group EH, 6.0 g·kg^-1 ·d^-1） and losartan potassium group（group LP, 30 mg ·kg^-1· d^-1）, 10 rats in each group. The rats of group EL, EM, EH and group LP were administrated by gavage. Rats of the other two groups were offered distilled water as much. Each group rats were administered continuously after 12 weeks, records of biochemical indicators and observes the renal pathological changes through HE and Masson dyeing, the immunohistochemical and western blot methods were used to observe the expression of UrotensinⅡ, collagen Ⅰ ,Ⅲ in renal tissues. Results. （1） As compared with Control group, DN group rats at the end of the 12 week, the glomerular of the rats became hypertrophic and mesangial matrix increased, interstitial collagen deposition, 24h urinary protein, serum BUN, Scr levels were significantly higher（ P 〈0.05）, U Ⅱ , collagen Ⅰ , Ⅲ expression increased （ P 〈0.05 ）. （2） Compared with group DN, the pathology changes were relieved, BUN, Scr, 24 h urinary protein as well as the expression of U Ⅱ, collagen Ⅰ , Ⅲ decreased in group EM and group EH （ P 〈0.05 ）. Conclusion： Euryale ferox may inhibit overexpression of U Ⅱ and the collagen Ⅰ , Ⅲ in renal interstitial, improve kidney function, delay diabetic nephropathy tubulointerstitial fibrosis, and thus play a role in renal protection.