血管内皮生长因子与肿瘤坏死因子-α在佐剂关节炎大鼠滑膜中表达

目的:探讨血管内皮生长因子(VEGF)和肿瘤坏死因子-α(TNF-α)在佐剂关节炎(AA)大鼠滑膜组织中的表达及其与关节病理积分的关系。方法:建立AA大鼠模型,常规HE染色,计算关节病理积分,并用免疫组织化学染色检测VEGF和TNF-α蛋白表达。结果:AA组大鼠滑膜VEGF和TNF-α蛋白表达在3周、8周及20周时均明显高于健康对照组(均P<0.01),且二者均与关节病理积分呈显著正相关(均P<0.01),二者之间亦呈显著正相关(均P<0.01)。结论:VEGF和TNF-α在关节炎的形成及发展过程中起重要作用,二者互相作用并影响滑膜新生血管的形成。

Expression of vascular endothelial growth factor and tumor necrosis factor-alpha in synovium of rats with adjuvant arthritis

AIM: To investigate the expression of vascular endothelial growth factor (VEGF) and tumor necrsis factor-alpha (TNF-α) in synovium of rats with adjuvant arthritis (AA) and the relationship between the pathological score and the expression of VEGF and TNF-α protein. METHODS: AA was produced in Wistar rats by inoculating complete Freund's adjuvant (CFA). The arthral pathological score was calculated, production of VEGF and TNF-α protein were assayed by histoimmunochemical staining at different stage after CFA inoculation. RESULTS: In AA group, the pathological score and expression of VEGF protein in synovium increased significantly (P＜0.01). There was significant positive correlation between the pathological score and protein expression of VEGF and TNF-α, respective (P＜0.01). CONCLUSIONS: VEGF and TNF-α may play an important role in the progress of arthritis. They interact with each other and affect the vascularization in the synovial tissue.