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| 应用PCR-SSP技术对云南拉祜族人HLA-DRB1进行基因分型 | |
| 引用本文： | Hong KX,He Q,San XY,Yu JK,Lin KQ,Shi L,Chu JY. 应用PCR-SSP技术对云南拉祜族人HLA-DRB1进行基因分型[J]. 中国医学科学院学报, 2002, 24(4): 389-392 |
| 作者姓名： | Hong KX He Q San XY Yu JK Lin KQ Shi L Chu JY |
| 作者单位： | 1. National Center for AIDS Prevention and Control, Beijing 100050 2. The Second Affiliated Hospital,Kunming Medical Colledge,Kunming 650118 3. The Prefecture Hospital of Xishuangbanna, Jinghong 666102 4. 中国医学科学院中国协和医科大学医学生物学研究所遗传室,昆明,650118 |
| 基金项目： | 国家自然科学基金重大项目(39993420),美国中华医学基金(CMB98-675)资助~~ |
| 摘 要： | 目的了解云南拉祜族健康人HLA-DRB1基因的遗传多态性。方法应用PCR-SSP技术对110名无亲缘关系的云南拉祜族健康人进行HLA-DRB1基因分型。结果鉴定了拉祜族群体HLA-DRB1位点的16种等位基因,包括DRB1位点目前已知的全部血清学特异性,经检验DRB1位点的基因型分布符合Hardy-Weinberg平衡(P>0.75)。结论提供了一套比较完整准确的云南拉祜族群体DRB1等位基因的基因频率,对群体遗传和疾病关联研究具有参考意义。 |
| 关 键 词： | HLA-DRB1 聚合酶链反应 序列特 异引物基因分型 拉祜族 |
| 修稿时间： | 2001-12-29 |
Genotyping of HLA-DRB1 by PCR-SSP in Yunnan Lahu ethnic groups |
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| Hong Kun-xue,He Qin,San Xi-yun,Yu Jian-kun,Lin Ke-qin,Shi Lei,Chu Jia-you. Genotyping of HLA-DRB1 by PCR-SSP in Yunnan Lahu ethnic groups[J]. Acta Academiae Medicinae Sinicae, 2002, 24(4): 389-392 | |
| Authors: | Hong Kun-xue He Qin San Xi-yun Yu Jian-kun Lin Ke-qin Shi Lei Chu Jia-you |
| Affiliation: | Department of Genetics, Institute of Medical Biology, CAMS, PUMC, Kunming 650118, China. |
| Abstract: | Objective To investigate polymorphism of HLA-DRB1in Chinese Lahu population in Yunnan.Methods Polymerase chain reaction-sequence specific primers(PCR-SSP)were used to determine HLA-DRB1genotypes of110unrelated healthy Lahu minority people of Yunnan Province.Results Sixteen alleles of DRB1were detected in this study.The results of test showed that the genotype distributions observed were corresponded with the Hardy-Weinberg equilibrium.Conclusion This study has obtained a more comprehensive and accurate data set of the normal allele frequencies of HLA-DRB1in Chinese Lahu population in Yunnan Province,which may be of significance in the studies on population genetics and disease association. |
| Keywords: | HLA-DRB1 polymerase chain reaction sequence specific primers genotyping Lahu Chinese |
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