4-氯-2-氟-3-甲氧基苯硼酸的急性毒性研究

目的探讨4-氯-2-氟-3-甲氧基苯硼酸的急性经口毒性,急性经皮毒性,急性皮肤刺激和急性眼刺激性.方法 按照《化学品测试方法》(国家环境保护总局,2004年)开展急性经口毒性试验,急性经皮毒性试验,急性皮肤刺激试验和急性眼刺激性试验. 结果①急性经口毒性试验:雌性大鼠急性经口LD₅₀为369mg/kg,95%可信区间为227mg/kg~599mg/kg;雄性大鼠急性经口LD₅₀为233mg/kg,95%可信区间为160mg/kg ~399mg/kg.对死亡大鼠解剖见肺脏充血.②急性经皮毒性试验:大鼠急性经皮LD₅₀ >2500mg/kg.③急性皮肤刺激试验:对大耳白兔染毒后各观察时点皮肤刺激反应总积分平均值和皮肤刺激反应时点最高积分均值均为0分.④急性眼刺激性试验:对大耳白兔眼刺激试验分级为5级.结论 4-氯-2-氟-3-甲氧基苯硼酸对雌雄性大鼠急性经口毒性分别属"低毒","中毒".靶器官可能是肺脏;对大鼠急性经皮毒性属"实际无毒";对大耳白兔皮肤不产生刺激性,但可对眼睛产生中等刺激性.

Study on acute oral toxicity,acute dermal toxicity,acute dermal irritation and acute eye irritation of 4-chloro-2-fluoro-3-methoxybenzeneboronic acid

Objective To study acute oral toxicity, acute dermal toxicity, acute dermal irritation and acute eye irritation of 4-chlorine -2- fluoro -3- methoxybenzeneboronic acid. Methods The tests were conducted in compliance with The Guidelines for the Testing of Chemicals. Results ①Acute oral toxicity test: LD₅₀ in female rats was 369mg/kg, confidence interval in 95% was 227mg/kg ~ 599mg/kg; LD₅₀ in male rats was 233mg/kg, confidence interval in 95% was 160mg/kg ~ 399mg/kg. Pulmonary congestion may be seen by anatomy on the dead rats. ②Acute dermal toxicity test:LD₅₀ in rats was more than 2500mg/kg. ③Acute dermal irritation test: Both mean values of the total integral and the highest integral in sometime for dermal irritation were "0" in each observation point,after albino rabbits exposed to acute dermal irritation. ④Acute eye irritation test: The grade classification of eye irritation test for albino rabbits was level 5. Conclusions Acute oral toxicity of 4- chloro -2- fluoro -3- methoxybenzeneboronic acid for female and male rats belongs to "low toxicity","moderate toxicity", respectively .Target organ may be lung;Acute dermal toxicity for rats is " non-toxic "; It is non dermal irritant to albino rabbits, but may be medium ocular irritant.