杭州湾南岸滩涂沉积物中POPs混合物对小鼠的生殖毒性作用

目的研究杭州湾南岸滩涂沉积物中POPs混合物对小鼠的生殖毒性作用. 方法选择健康清洁级性成熟ICR小鼠80只,体重22-26g,随机分为30,100,300mg/kg三个剂量染毒组和一个溶剂对照组,每组20只,雌雄各半,进行一代生殖毒性试验.采用灌胃方式进行染毒(10 ml/kg),雄鼠染毒6周,进行精子畸形测定.雌鼠染毒2周后,雌雄小鼠按1:1同笼交配,交配时间为2周,观察交配率及受孕率.孕鼠在妊娠19天解剖,观察黄体数,着床数,活胎数,死胎数,吸收胎数,每窝胎仔平均胎长,胎重,胎盘重.结果 当混合物染毒剂量为100 mg/kg和300mg/kg 时,精子畸形率明显升高(P<0.05),剂量达到300mg/kg时生育率,妊娠率,活胎率,平均胎长和胎仔重降低(P<0.05),而着床前死亡率,死胎率升高(P<0.05).结论 滩涂沉积物中POPs混合物在剂量达到300mg/kg时对小鼠产生明显的生殖和发育毒性.

Effects of persistent organic pollutants mixtures in tidal-flat sediment of south of Hangzhou Bay on Reproductive toxicity of Mice

ObjectiveTo study the Reproductive toxicity of typical persistent organic pollutants mixtures in tidal-flat sediment of south of Hangzhou Bay in mice.Methods80 mature ICR mice(clean anima,sexual maturation,weighed 22-26g)were randomly into three experimental groups(30,100 and 300 mg/kg ) and the control group,20 in team,the reproductive toxicity trial in mice.Which all exposed by intragastric administration(10 ml/kg), male mice were gavage administration for 6 consecutive weeks, for the determination of sperm deformity.Female mices were gavage administration for 2 weeks,and then mated with male mices (male: female, 1∶1).The time of mating of two weeks, detected Copulation rate and pregnancy rate.Pregnant mice were dissected on 19 day,detected number of corpora lutea resorptions,live and dead fetuses and implantations,detected length of fetus and fetal weight and placental weight.ResultsWhen the doses of typical persistent organic pollutants were 100 and 300 mg/kg,sperm vitality rate was reduce and rate of sperm deformation was hoist,there had obvious difference compared with control group (P<0.05).When the doses of typical persistent organic pollutants was 300 mg/kg,fertility rate and pregnancy rate and live fetuses rate and length of fetus and fetal weight were reduce (P<0.05),while rate of pre-implantation death and dead fetuses was hoist(P<0.05).ConclusionsEffects of persistent organic pollutants mixtures was 300 mg/kg in tidal-flat sediment have a marked reproductive and developmental toxicity for mice.