运动神经端侧缝合后再生及趋化性的实验研究

目的探讨运动神经端侧缝合后神经再生及其趋化性问题。方法将20只健康雌性SD大鼠分为神经端侧缝合组(端侧缝合组)和对照组,各10只。端侧缝合组切断大鼠胸前神经分支,将尺神经后内侧方外膜开窗,将胸前神经分支和开窗的尺神经外膜做端侧缝合;对照组仅显露胸前神经分支和尺神经。两组1年后均采用乙酰胆碱酯酶染色和Fluoro-Gold荧光逆行示踪技术,观察和计数胸前神经分支纤维和大鼠脊髓C5-T1前角和背根神经节神经元。结果端侧缝合组有髓神经纤维数、轴突酶染阳性的运动神经纤维数、运动纤维比例、脊髓前角神经元数目、背根神经节感觉神经元数目、神经元总数和运动神经元比例与正常组比较,差异均有统计学意义(<0.01或<0.05)。结论运动神经端侧缝合后神经侧方出芽对特定靶器官的趋化性不明显。

An experimental study on neurotropism following End-To-Side Neurorrhaphy of motor nerve

Objective To investigate neurotropism following end-to-side neurorrhaphy of motor nerve.Methods T wenty female Sprague-Dawley rats were randomly divided into 2 groups: the end-to-side neurorrhaphy group and the control group.In the end-to-side group ,the branch of pectoralis nerve was transected, then an 1mm epineural window was created on the ulnar nerve. Distal end of pectoralis nerve was sutured to the windowed ulnar nerve by means of end-to-side neurorrhaphy.In the control group,the branch of pectoralis nerve and ulnar nerves were exposed. One year after operation, acetylcholinesterase-stain and retrograde Fluoro-Gold neuron tracing techniques, the numbers of pectoralis nerve fibers and C5-T1 anterior horn motoneuron and dorsal root ganglion sensory neuron of all groups were counted.R esult The motor fiber counts in pectoralis nerve end-to-side group were 29.4% of normal control group (P<0.01),and the difference of the ratio of motor fibers between end-to-side group and normal control group was significant (P<0.01). The motor neuron counts in pectoralis nerve end-to-side group were 23.9% of normal control group (P<0.01), and the difference of the ratio of motor neurons between end-to-side group and normal control group was significant (P<0.01).Conclusion Neurotropism in collateral spouting after end-to-side neurorrhaphy of motor nerve is not significant.