外源性碱性成纤维细胞生长因子对人腺样囊性癌细胞株ACC-2增殖和细胞外信号调节激酶、Cyclin D1及p21waf/cip1通路的影响

目的腺样囊性癌（ACC）是最常见的唾液腺恶性肿瘤之一。本研究拟观察碱性成纤维细胞生长因子
（bFGF）对人ACC- 2细胞增殖及细胞外信号调节激酶（ERK）、Cyclin D1及p21waf/cip1信号通路的影响。方法培养人
ACC- 2细胞，MTT比色法测定不同浓度的外源性bFGF对细胞增殖的影响；采用ERK活性测定试剂盒测定ERK活性；
免疫印迹法测定p- ERK1/2和下游的Cyclin D1及p21waf/cip1表达。并观察丝裂原蛋白活化激酶激酶（MEK）抑制剂U0126对
上述指标的干预作用。结果MTT实验显示bFGF明显增强ACC- 2细胞增殖，bFGF可上调ERK活性，免疫印迹法显
示bFGF明显增强p- ERK1/2、Cyclin D1表达及抑制p21waf/cip1表达。U0126可抑制bFGF的以上效应。结论bFGF可促进
人ACC- 2细胞增殖，其途径与上调p- ERK1/2活性、抑制p21waf/cip1表达进而增强Cyclin D1表达有关。本研究为ACC的发
病机制及治疗提供新思路。

Effects of basic fibroblast growth factor on the proliferation of human salivary adenoid cystic carcinoma cell line ACC-2 and extracellular signal-regulated kinase,Cyclin D1,p21waf/cip1 signaling pathway

Objective To investigate the effects of basic fibroblast growth factor(bFGF) on the proliferation of human salivary adenoid cystic carcinoma(ACC) cell line ACC-2 in vitro.Methods The effect of ectogenic bFGF on proliferation of ACC-2 was observed by MTT assay.Extracellular signal-regulated kinase(ERK) activity was measured by immuno-precipitation.p-ERK1/2,Cyclin D1 and p21waf/cip1 expression were assessed by Western blot.Results bFGF could enhance the proliferation of ACC-2.Stimulated by bFGF,the proliferation ratio increased significantly.The intracellular ERK activity,p-ERK1/2 and Cyclin D1 expression were increased,while p21waf/cip1 expression was inhibited by different concentrations of bFGF.The above effects of bFGF could be attenuated by MEK inhibitor U0126.Conclusion bFGF stimulates the proliferation of ACC-2 in a dose dependent manner.The proliferation effect of bFGF may be due to up-regulating ERK,Cyclin D1 and p21waf/cip1 signaling pathway.This research can help us to explore a new pathogenesis and therapy of the ACC.