电针ST36穴位治疗牛黄胆酸钠诱导的急性重症胰腺炎的疗效观察

Objective:To explore the protective effects and mechanisms of electroacupuncture(EA)at Zusanli(ST36)acupoint in rats with severe acute pancreatitis(SAP).Methods:Sixty-six male SpragueDawley rats were randomly assigned to three groups of 22 each:a SAP model group(SAP group),a shamoperated group(sham group)and a EA at ST36 acupoint group(EA group).A rat model of SAP was induced by pancreatic duct injection with 3.5%sodium taurocholate.EA was performed at ST36 acupoint for 30 min after induction of SAP and 30 min before sacrificed.The rats were killed at 3 h(n=7),6 h(n=7)and 12 h(n=8)after operation,and blood samples were taken for the measurement of tumor necrosis factor-alpha(TNF-a),interleukin-6(IL-6)and acetylcholine(Ach)by enzyme-linked immunosorbnent assay.The pathological changes of pancreatic tissue,volume of ascites and pancreatic weight/body weight ratio were measured.Results:The serum concentrations of TNF-αand IL-6 in the EA group were significantly lower than in the SAP group at 3,6and 12 h after the operation(P0.05).Serum Ach in the EA group was significantly higher than in the SAP group at various time points after operation(P0.05).The other parameters were clearly improved after treatment with EA.Conclusion:EA at ST36 acupoint might have a therapeutic effect in rats with SAP through activating the cholinergic anti-inflammatory pathway.

Therapeutic Effects of Electroacupuncture at ST36 Acupoint on Sodium-Taurocholate-Induced Severe Acute Pancreatitis

severe acute pancreatitis, electroacupuncture, ST-36, cholinergic anti-inflammatory pathway