| 血小板激活因子前体Lyso-PAF的生物测定法及在烫伤大鼠渗出液中的含量改变 |
| |
| 引用本文： | 殷明,方之扬. 血小板激活因子前体Lyso-PAF的生物测定法及在烫伤大鼠渗出液中的含量改变[J]. 第二军医大学学报, 1988, 0(6) |
| |
| 作者姓名： | 殷明 方之扬 |
| |
| 作者单位： | 长海医院烧伤科(殷明)，长海医院烧伤科(方之扬) |
| |
| 摘 要： | 大鼠烫伤渗出液经有机溶剂萃取得到脂质部分,用乙酰化试剂使Iyso-PAF转变为PAF,再将该提取物的致血小板聚集作用与合成的PAF引起聚集的程度对比以定量。提取物经3种方法鉴定证实含有PAF。测定灵敏度可达10pg水平。提示对测定体液及渗出液中lyso-PAF的含量进而研究其意义是一种简便可行的方法。烫伤早期渗出液中lyso-PAF的含量升高可能对解释通透性变化等具有意义。
|
| 关 键 词： | 血小板激活因子 血小板聚集 烫伤 |
MEASUREMENT OF LYSO-PAF AND CHANGES OF ITS CONTENTS IN EXUDATE OF SCALDED RATS |
| |
| Yin Ming,et al. MEASUREMENT OF LYSO-PAF AND CHANGES OF ITS CONTENTS IN EXUDATE OF SCALDED RATS[J]. Former Academic Journal of Second Military Medical University, 1988, 0(6) |
| |
| Authors: | Yin Ming et al |
| |
| Abstract: | It is the purpose to set up a method to measure lyso-platelet-activating factor (PAF) and to observe the changes of its contents in exudate of scalded rats. The cxudate was extracted with organic solvents and the lipid portion was obtained. After acetylating, the effect of the transformed PAF on platelet aggregation was compared with that induced by standard PAF for quantification. Three kinds of characterizations confirmed the existence of lyso-PAF in the exudate. The increase of lyso-PAF in exudate of the scalded rats was observed.These results suggest that with this simple method the concentrations of lyso-PAF in body fluids could be detected and the increase of lyso-PAF in the exudate may be responsible for the changes of vasopermeability in the early period of burn. |
| |
| Keywords: | platelet-activating factor platelet aggregation burn |
| 本文献已被 CNKI 等数据库收录！ |
