bFGF和硫糖铝在持续恒压扩张术中局部联合应用对组织细胞增殖的影响

目的 研究bFGF和硫糖铝在扩张术中局部应用对细胞增殖的影响。方法 白色小猪9只，自身对照，分为实验Ⅰ、Ⅱ、Ⅲ组，对照组，扩张术中分别注入bFGF和硫糖铝、bFGF、硫糖铝、生理盐水。各动物于术后第1～14天取出扩张器后6周取材作免疫组织化学检查。结果 持续恒压扩张术同时加用bFGF和硫糖铝后，表皮层基底细胞增殖更早更明显，尤其以第3天最为显著，14d基底细胞增殖指数最低但也高于对照组（持续恒压扩张组）；真皮层成纤维细胞，毛细血管内皮细胞，毛囊上皮细胞，汗腺上皮细胞增殖更明显，早于对照组，7d达高峰，14d明显减少但仍高于对照组，6周基本恢复正常。实验Ⅱ、Ⅲ组与对照组差异无显著性意义。结论 bFGF和硫糖铝联合局部应用于扩张术，促细胞增殖作用强，从而在扩张早期有效地诱导皮肤细胞分裂增殖，促进皮肤软组织生物性生长。

The effect of bFGF and sucralfate on cell proliferation during continuous tissue expansion

Objective To investigate the effect of local application of bFGF combined with sucralfate on the cell proliferation during continuous tissue expansion (CTE). Methods Nine white pigs were selected to undergo the continuous tissue expansion in this study and treated with bFGF and sucralfate , respectively as the following groups: group 1 with both bFGF and sucralfate , group 2 only with bFGF , group 3 with only sucralfate, and group 4 with saline as control. Fifteen samples were taken in each pig for immunohistochemistry analysis 1-14 days and 6 weeks after the operation. Results In the group with both bFGF and sucralfate, the epidermic basal cells proliferated significantly after the operation and reached top level in 3 days, which was statistical higher than the control guoup, but the multiplication of basal cell was the lowest 14 days after the operation, still more than the control guoup. In dermal layer, proliferation of fibroblasts,vessel endothelial cells,hair follicles epidermic cells and sweat gland epicytes was also significant higher in the group with both bFGF and sucralfate than that the control group and reached top level 7 day after the operation, but the proliferation of cells decreased obviously 14 days after the operation, still higher than the control group .The mitotic activity of cells returned to the basal level in 42 days . There were no significant differences among the group 2, group 3 and group 4. Conclusion Local application of both bFGFand sucralfate could be more effect to induce cells multiplication during early skin expansion to facilitate the growth of neoformed skin soft tissue.;