| 血红素氧合酶-1对移植入急性梗死区心肌骨髓间充质干细胞的保护作用 |
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| 引用本文： | 曾彬,付金蓉,马乐乐,易欣,张静.血红素氧合酶-1对移植入急性梗死区心肌骨髓间充质干细胞的保护作用[J].微循环学杂志,2011,21(4):10-12,7,1,4. |
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| 作者姓名： | 曾彬 付金蓉 马乐乐 易欣 张静 |
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| 作者单位： | 武汉大学人民医院心内科,武汉,430060 |
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| 基金项目： | 国家自然科学基金青年基金 |
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| 摘 要： | 目的:观察血红素氧合酶-1(HO-1)体内外对缺血环境下骨髓间充质干细胞(MSCs)的保护作用及梗死后心脏血流动力学的影响。方法:取大鼠骨髓,体外分离扩增培养MSCs;HO-1腺病毒转染MSCs,荧光显微镜下观察绿色荧光蛋白(GFP)的表达;流式细胞仪检测无氧无血清条件培养的HO-1-MSCs、MSCs的凋亡率。建立心梗模型,分别将DAPI-HO-1-MSCs、DAPI-MSCs多点注射到大鼠心脏梗死区周边,对照组注射等量PBS,比较各组MSCs存活率;4周后测定血流动力学指标。结果:HO-1转染的MSCs可稳定高效地表达GFP;在无氧无血清条件下,HO-1-MSCs的凋亡率显著低于MSCs(P<0.01);细胞移植到梗死后心肌,HO-1-MSCs组存活MSCs计数在第7天明显高于MSCs组(P<0.05),且血流动力学各项指标较MSCs组明显改善(P<0.05)。结论:HO-1可在体内、体外实验中提高缺血环境下MSCs的存活率,并改善心肌梗死后血流动力学和心功能,为优化干细胞治疗提供了新思路。
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| 关 键 词： | 骨髓间充质干细胞 急性心肌梗死 血红素氧化酶-1 凋亡 |
The Protective Effects of Mesenchymal Stem Cells Transplanted into Acute Myocardial Infarction Heart by Human Heme Oxygenase-1 |
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| Zeng Bin,Fu Jinrong,Ma Lele,et al.The Protective Effects of Mesenchymal Stem Cells Transplanted into Acute Myocardial Infarction Heart by Human Heme Oxygenase-1[J].Chinese Journal of Microcirculation,2011,21(4):10-12,7,1,4. |
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| Authors: | Zeng Bin Fu Jinrong Ma Lele |
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| Institution: | Zeng Bin,Fu Jinrong,Ma Lele,et al Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060 |
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| Abstract: | Objective:To investigate the effects of mesenchymal stem cells(MSCs) transfected with heme oxygenase-1(HO-1) gene on its survival under situtation of serum-free and hypoxia in acute myocardial infarction(AMI) heart.Method:MSCs were acquired from the bone marrow of adults rats.They were isolated,purified,cultured,and transfected with Adv-HO-1.The expression of GFP and cell apoptosis were detected by immunofluorescence;Cell apoptosis was detected by FACS respectively.The number of DAPI-positive cells was eval... |
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| Keywords: | Mesenchymal stem cells Acute myocardial infarction Heme oxygenase-1 Apoptosis |
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