| 肌球蛋白调节性轻链在慢性心力衰竭大鼠心室肌的表达变化 |
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| 引用本文： | 吴钢,黄从新,江洪,陈静茹,徐欣. 肌球蛋白调节性轻链在慢性心力衰竭大鼠心室肌的表达变化[J]. 微循环学杂志, 2011, 21(4): 17-18,22,7,10,4 |
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| 作者姓名： | 吴钢 黄从新 江洪 陈静茹 徐欣 |
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| 作者单位： | 1. 武汉大学人民医院心内科,武汉,430060
2. 华中科技大学生命科学院 |
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| 摘 要： | 目的:检测肌球蛋白调节性轻链(MYL2)在慢性心衰大鼠心室肌的表达变化。方法:建立腹主动脉缩窄大鼠慢性心衰模型,采用免疫组化染色和集成光学密度(IOD)定量观测MYL2在心室肌的表达及其表达程度,并与假手术组对照分析。结果:心衰组大鼠左室收缩末压(LVSP)、左室内压上升最大速率(+dp/dtmax)、左室内压下降最大速率(-dp/dtmax)显著低于假手术组,左室舒张末压(LVEDP)显著高于假手术组(P均<0.01)。免疫组化染色示心衰组心室肌MYL2表达显著低于假手术组;心衰组染色标本的平均IOD为(47.12±12.08)×106,假手术组为(97.77±36.21)×106,前者显著低于后者(P<0.01)。结论:心室肌MYL2表达的显著降低可能是慢性心衰发生的重要机制。
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| 关 键 词： | 慢性心力衰竭 肌球蛋白调节性轻链 大鼠 动物模型 |
Change of Expression Level of Myosin Regulatory Light Chain in Cardiocytes with Chronic Heart Failure |
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| Wu Gang,Huang Congxin,Jiang Hong,et al. Change of Expression Level of Myosin Regulatory Light Chain in Cardiocytes with Chronic Heart Failure[J]. Chinese Journal of Microcirculation, 2011, 21(4): 17-18,22,7,10,4 |
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| Authors: | Wu Gang Huang Congxin Jiang Hong et al |
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| Affiliation: | Wu Gang,Huang Congxin,Jiang Hong,et al Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060 |
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| Abstract: | Objective:To detect the change of expression level of myosin regulatory light chain(MYL2) in chronic heart failure(CHF).Method:Rat model of CHF of abdominal aortic banding was established.Immunohistochemistry stain was carried on rat ventricular myocytes sample.Integrated optical density(IOD) was detected to determine the expression of MYL2.Results:The left ventricular end-systolic pressure(LVSP),the maximum increase rate of left ventricular pressure(+dp/dtmax),the maximum decrease rate of left ventricular ... |
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| Keywords: | Chronic heart failure Myosin regulatory light chain Rat Animal model |
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