| 茶多酚对2型糖尿病患者外周血早期内皮祖细胞衰老及氧自由基损伤的影响 |
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| 引用本文： | 王颖超,王晓霞,赵明. 茶多酚对2型糖尿病患者外周血早期内皮祖细胞衰老及氧自由基损伤的影响[J]. 微循环学杂志, 2011, 21(4): 19-22,7,11,78 |
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| 作者姓名： | 王颖超 王晓霞 赵明 |
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| 作者单位： | 1. 南京大学医学院临床学院,南京军区南京总医院内分泌科,南京210002
2. 南京军区南京总医院干部内分泌科 |
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| 摘 要： | 目的:通过体外诱导培养2型糖尿病(T2DM)患者外周血早期内皮祖细胞(earlyEPC),观察茶多酚(TP)对其衰老和氧自由基损伤的影响。方法:分别采集20例T2DM患者及15例正常对照人群外周血各20ml,获取单个核细胞后,在包被有纤维连接蛋白(FN)的培养板中加入含有血管内皮生长因子(VEGF)等的M199培养基,诱导earlyEPC分化,于第6天加入不同浓度TP干预24h后,测定earlyEPC衰老情况及丙二醛(MDA)含量。结果:两组样本分别给予TP(1mg/L、10mg/L、100mg/L)干预后,earlyEPC的衰老细胞明显减少(P<0.01),MDA含量明显降低(P<0.05),但TP对实验组earlyEPC的衰老和MDA改善情况不如对照组明显(P<0.05);100mg/L的TP对T2DM患者MDA的改善效果不明显(P>0.05)。结论:适宜浓度TP能够不同程度改善T2DM患者earlyEPC的衰老及氧自由基损伤。
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| 关 键 词： | 2型糖尿病 早期内皮祖细胞 茶多酚 丙二醛 |
Tea Polyphenals on the Impact of the Cell Senescence and Oxygen Free Radical Damage of Early Endothelial Progenitor Cells from Type 2 DM Peripheral Blood |
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| Wang Yingchao,Wang Xiaoxia,Zhao Ming. Tea Polyphenals on the Impact of the Cell Senescence and Oxygen Free Radical Damage of Early Endothelial Progenitor Cells from Type 2 DM Peripheral Blood[J]. Chinese Journal of Microcirculation, 2011, 21(4): 19-22,7,11,78 |
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| Authors: | Wang Yingchao Wang Xiaoxia Zhao Ming |
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| Affiliation: | Wang Yingchao,Wang Xiaoxia,Zhao Ming Department of Endocrinology,School of Medicine,Nanjing University/Nanjing General Hospital of Nanjing Military Command,PLA,Nanjing 210002 |
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| Abstract: | Objective:To study the culture method of early endothelial progenitor cells(EPCs) from type 2 diabetes peripheral blood in vitro and the impact of tea polyphenals(TP) on the early EPCs senescence and oxygen free radical damage.Method:MNCs(mononuclear cells) were isolated from patients with type 2 diabetes(n=20) and age-matched healthy control subjects(n=15),and then the cells were plated on fibronectin-coated culture dishes in M199 medium in the presence of vascular endothelial growth factor which can induc... |
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| Keywords: | Type 2 diabetes early EPCs Tea polyphenals MDA |
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