| 5/6肾切除大鼠肾脏微循环改变 |
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| 引用本文： | 马林林,解汝娟. 5/6肾切除大鼠肾脏微循环改变[J]. 微循环学杂志, 2011, 21(4): 13-16,7,10,77 |
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| 作者姓名： | 马林林 解汝娟 |
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| 作者单位： | 哈尔滨医科大学附属第一医院肾内科,哈尔滨,150001 |
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| 摘 要： | 目的:观察5/6肾切除大鼠肾脏微循环的改变。方法:实验分为假手术组和5/6肾切除组,平行检测24h尿蛋白、尿蛋白/尿肌酐比值和血肌酐;观察肾组织病理学变化;免疫组化法观察小鼠抗大鼠氨肽酶P(JG-12)染色情况;检测肾组织内皮型一氧化氮合酶(eNOS)和组织型纤溶酶原激活物(tPA)mRNA和蛋白质的表达。结果:5/6肾切除组大鼠出现尿蛋白增多、尿蛋白/尿肌酐比值增加及血肌酐升高;肾组织可见肾小球硬化、肾小管萎缩和间质纤维化;免疫组化示毛细血管密度降低;eNOSmRNA及蛋白质表达减少,tPAmRNA及蛋白质表达增加。结论:5/6肾切除大鼠存在肾微循环障碍及血管内皮损伤。
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| 关 键 词： | 微循环 血管内皮 5/6肾切除 大鼠 |
The Change of Renal Microcirculation in 5/6 Nephrectomy Rats |
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| Ma Linlin,Xie Rujuan The First Affiliated Hospital of Harbin Medical University,Harbin. The Change of Renal Microcirculation in 5/6 Nephrectomy Rats[J]. Chinese Journal of Microcirculation, 2011, 21(4): 13-16,7,10,77 |
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| Authors: | Ma Linlin Xie Rujuan The First Affiliated Hospital of Harbin Medical University Harbin |
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| Affiliation: | Ma Linlin,Xie Rujuan The First Affiliated Hospital of Harbin Medical University,Harbin 150001 |
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| Abstract: | Objective:To investigate the change of renal microcirculation in 5/6 nephrectomy rats.Method:Rats were divided into sham operation group and 5/6 nephrectomy group.24-hour urinary protein,urinary protein/urinary creatinine ratio and serum creatinine were parallel measured.Renal pathological changes were observed.The staining of mouse anti-rat aminopeptidase P in kidneys was observed by immunohistochemical method.The expression of endothelial nitricoxide synthase(eNOS) and tissue plasminogen activator(tPA) in... |
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| Keywords: | Microcirculation Endothelium 5/6 nephrectomy Rat |
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