经腹膜外盆腔淋巴间隙给药对妇科恶性肿瘤患者NTIL细胞的影响

目的 :探讨通过盆腔腹膜外淋巴间隙进行肿瘤生物治疗的可行性。方法 :妇科恶性肿瘤患者 4 1例分为IL 2组 (n =10 )、IL 2 + 5 FU组 (n =11)、5 FU组 (n =10 )及对照组 (n =10 ) ;通过经盆腔腹膜外淋巴间隙留置管 ,分别予以IL 2、IL 2 + 5 FU、5 FU治疗 ,在手术中摘取淋巴结 ,分离淋巴细胞 ,用流式细胞仪检测T细胞表面标记 ,CD2 5活化标记变化及NK细胞数量。结果 :治疗后各组淋巴结CD3+,CD4 +,CD8+,CD2 5 +,NK细胞数量均明显高于对照组 (P均 <0 .0 1) ;IL 2 + 5 FU组CD3+,CD4 +,CD8+,CD2 5 +及NK细胞含量均明显高于 5 FU组 (P <0 .0 5 ) ;IL 2组NK细胞含量明显高于 5 FU组 (P <0 .0 5 )。结论 :经盆腔腹膜外淋巴间隙进行生物治疗能有效激活机体局部淋巴结中T细胞反应 ,促进T细胞增殖及增加NK细胞数量

Effects on the NTIL of drugs injected via pelvic retroperitoneal space in gynecological cancer patients

Objective:To explore the feasibility and effect of biotherapy instituted via pelvic retroperitoneal space on gynecologic cancer.Methods:IL 2 or/and 5 FU were injected through a tube installed at the pelvic retroperitoneal space.The subpopulation of T cell and NK of lymphnodes of pelvic were counted by FCM after given drugs.Results:The numbers of CD3 +,CD4 +,CD8 +,CD25 + and NK cells in treatment group were significantly higher than those in the control group,and the numbers of these cells in IL 2+5 FU group were significantly higher than those in the 5 FU group,the NK cell numbers in the IL 2 group were significantly higher than those in the 5 FU group.Conclusion:IL 2 injected via pelvic retroperitoneal space can promote the activity and development of T and NK cell in the lymphnodes of pelvic.