慢性支气管炎患者自然杀伤T细胞的表达与临床意义

目的:探索自然杀伤T细胞(NKT)在慢性支气管炎患者的表达及意义。方法:收集慢性支气管炎患者30例(慢支组),以30例健康志愿者为对照组;采用流式细胞术分析NKT及其胞内细胞因子γ干扰素(IFN-γ)及肿瘤坏死因子α(TNF-α)的含量。结果:与对照组比较,慢支组NKT及IFN-γ含量明显降低(P0.05),但TNF-α含量明显升高。结论:NKT及IFN-γ含量降低、TNF-α含量增加,是慢性支气管炎的一个重要免疫学现象,可能是慢性炎症导致TNF-α蓄积,而NKT及IFN-γ消耗过多所致。

Expression and significance of natural killer T cells in patients with chronic bronchitis

Objective:To explore the expression and significance of natural killer cells( NKT) for patients with chronic bronchitis. Methods: Thirty patients with chronic bronchitis were enrolled as the observation group,and 30 healthy volunteers were enrolled as the control group. The levels of NKT and its intracellular cytokines,including the gamma interferon( IFN-γ) and tumor necrosis factor alpha( TNF-α),were detected by using flow cytometry analysis. The levels of NKT,IFN-γ and TNF-α between the two groups were compared.Results: The percentages of NKT,IFN-γ and TNF-α in the patients with chronic bronchitis were( 7. 31 ±2. 09) %,( 3. 28 ± 1. 01) % and( 11. 63 ± 6. 25) %,whereas the percentages of NKT,IFN-γ and TNF-α in the healthy volunteers were( 18. 16 ± 4. 57) %,( 10. 24 ± 3. 69) % and( 4. 38 ± 1. 87) %. The deference of NKT,IFN-γ and TNF-α between the two groups was statistically significant( all P < 0. 0001). Conclusion:Our findings show that reducing of NKT and IFN-γ,as well increasing of TNF-α,could be a vital immunological feature for patients with chronic bronchitis. The possible reason for this important phenomenon could be: the chronic inflammation in the bronchitis cause the accumulating of TNF-α,and the excessive consumption of NKT and IFN-γ.