VEGF siRNA及bFGF siRNA对胰腺癌细胞增殖和侵袭性的影响

目的 探讨胰腺癌细胞中VEGF及bFGF的表达水平与其恶性生物学行为的关系.方法 将胰腺癌细胞株SW1990、Panc-1及PCT-3分别分为4组:对照组、VEGF siRNA处理组、bFGF siRNA处理组和VEGF siRNA+bFGF siRNA处理组.通过CCK-8法及Boyden Chamber趋化小室检测不同处理对3株细胞侵袭性及增殖能力的影响.结果 VEGF siRNA和bFGF siRNA能显著抑制3 株胰腺癌细胞中VEGF及bFGF的表达.CCK-8法检测结果显示:除PCT-3细胞外,其他两株细胞各处理组肿瘤细胞的增殖能力均弱于对照组(P＜0.05).Boyden Chamber趋化小室检测结果显示:3 株胰腺癌细胞各处理组与对照组比较,侵袭性的差异均有统计学意义(P＜0.05).结论 通过下调VEGF及bFGF的表达水平可抑制胰腺癌细胞的增殖与侵袭能力.

The significance of VEGF siRNA and bFGF siRNA in invasion and proliferation of pancreatic carcinoma cell

Objective To investigate the relationship between expression of angiogenic factors and invasion and proliferation of pancreatic cancer cell.Methods The three pancreatic cancer cell lines of SW1990,Panc-1 and PCT-3 were divided into four groups respectively:concrol group,VEGF siRNA group,bFGF siRNA group and VEGF siRNA + bFGF siRNA group.The expression and the secretion of VEGF and bFGF in the three cell lines were inhabited by VEGF siRNA and bFGF siRNA.The proliferation and the invasion of the three cell lines were determined by CCK-8 and Boyden Chamber invasion tests.Results Expressions of VEGF and bFGF in three cell lines were significantly inhibited by VEGF siRNA and bFGF siRNA.The proliferation was inhabited by VEGF siRNA and bFGF siRNA in SW1990 and Panc-1(P＜0.05),while wasn't in PCT-3(P＞0.05).The invasion was inhabited significantly by VEGF siRNA and bFGF siRNA in the three cell lines(P＜0.05).Combination of VEGF siRNA and bFGF siRNA resulted in more efficient influence in inhibition of invasion in Panc-1 and proliferation in PCT-3 and SW1990 than VEGF siRNA or bFGF siRNA individually.Conclusion The decreased expression of VEGF and bFGF can inhabited the ability of invasion and proliferation of pancreatic cancer cell