Development of cell therapy using autologous bone marrow cells for liver cirrhosis |
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Authors: | Isao Sakaida Shuji Terai Hiroshi Nishina Kiwamu Okita |
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Institution: | (1) Department of Gastroenterology & Hepatology, Yamaguchi University School of Medicine, 1-1-1 Minami Kogushi, Ube 755-8505, Japan;(2) Department of Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan |
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Abstract: | The plasticity of bone marrow has been confirmed by the autopsy of a female recipient of bone marrow cell transplantation
from a male donor. To establish new clinical cell therapies using autologous bone marrow cells for patients with liver failure,
we developed a new in vivo model named the green fluorescent protein (GFP)/carbon tetrachloride (CCl4) model. Using the GFP/CCl4 model, we found that transplanted Liv8-negative cells efficiently repopulated into cirrhotic liver tissue and differentiated
into albumin-producing hepatocytes under persistent liver damage induced by carbon tetrachloride. Moreover, bone marrow cell
transplantation into mice with liver cirrhosis improved liver function and liver fibrosis with the strong expression of matrix
metalloproteinases (MMPs), especially MMP-9 activity, resulting in an improved survival rate. Results from the GFP/CCl4 model showed that cell therapy using autologous bone marrow cells has the potential to become an effective treatment for
patients with liver failure. A summary of findings from the GFP/CCl4 model is described. |
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Keywords: | Bone marrow cell (BMC) Liver cirrhosis GFP (green fluorescent protein) Liver fibrosis Stem cell Liv8 |
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