Flow cytometric quantitation of the expression of membrane cofactor protein as a marker for the human sperm acrosome reaction. |
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| Authors: | O J D'Cruz G G Hass |
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| Affiliation: | Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City 73190. |
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| Abstract: | A mAb (TRA-2-10, IgG1) to an embryonal carcinoma cell line (2102ep) that recognizes an antigen termed membrane cofactor protein (CD 46, TLX antigen) binds to human sperm after chemical induction of acrosomal loss by Ca2+ ionophore. An indirect immunofluorescence assay was developed in which sperm membrane cofactor protein was detected by flow cytometry on acrosome-reacted living human sperm. The expression of the membrane cofactor protein antigen on acrosome-reacted sperm may represent a marker that can be used in a rapid, quantitative, and reproducible flow cytometric assay for the evaluation of human sperm acrosomal loss. |
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