骨形态发生蛋白-7在缺血和缺氧性损伤尾壳核细胞内的表达

目的 研究内源性骨形态发生蛋白-7(BMP-7)在缺血缺氧性损伤神经组织的表达情况,探讨内源性BMP-7在脑缺血中对损伤神经组织的保护作用.方法 制作大鼠局灶性脑缺血模型,用免疫组织化学方法检测大鼠脑缺血再灌注24h后和原代培养尾壳核细胞缺氧复氧状态下BMP-7的表达.用计算机图像分析系统测量免疫阳性产物的吸光度值,并进行统计学分析.结果 大鼠在脑缺血再灌注24h后,其缺血侧BMP-7尾壳核较非缺血侧表达明显增高且范围扩大,缺血侧平均吸光度值与非缺血侧比较有显著性差异(P<0.01);而在正常对照组和假手术组均未检测到双侧尾壳核内BMP-7的表达.原代培养尾壳核细胞缺氧复氧24h后神经元胞浆内出现BMP-7的阳性产物,但表达较弱,而正常尾壳核细胞未见BMP-7表达,结论缺氧缺血可引起内源性BMP-7的表达.提示内源性BMP-7对缺血缺氧性损伤的神经组织具有一定的保护作用.

THE EXPRESSION OF BONE MORPHOGENETIC PROTEIN-7 IN THE HYPOXIC AND ISCHEMIC CAUDAL-PUTAMEN CELLS OF RATS

Objective To study the expression of bone morphogenetic protein-7(BMP-7) in the hypoxic and ischemia damage striatum tissues,and investigate the neuroprotective effect of endogenous BMP-7 in the injuried nerve tissues of rats. Methods Rat models of focal cerebral ischemia were made.Immunohistochemistry method was used to detect the expression of BMP-7 in the rat striatum 24 hours after ischemia/reperfusion and caudal-putamen cells were cultured under hypoxia/reoxygenation. The absorbance of the positive products of the stained section was measured by computer image processing software,and statistics analysis was done. Results The expression of BMP-7 in the caudal-putamen was higher and more extensive in the ischemic side than that in the nonischemic side 24 hours after ischemia/reperfusion,and there was a statistically significant difference between the average absorbance in the ischemic side and that in the nonischemic side.(P<0.01),while BMP-7 expression was not detected in the bilateral caudal-putamen in the normal and sham control rat groups.In the primary cultural cells,the weak expression of BMP-7 was detected in the plasm of caudal-putamen cells 24 hours after hypoxia/reoxygenation,while no BMP-7 positive expression was found in the normal control caudal-putamen.Conclusion This experiment explained that hypoxic-ischemic can cause endogenous BMP-7 expression.The increased expression of BMP-7 indicated that endogenous BMP-7 may play a protective role in the injured nerve cells.