外周血T淋巴细胞亚群检测在胃癌病情监测及预后评价中的意义

目的：探讨外周血T淋巴细胞亚群检测在胃癌病情监测及预后评价中的价值。方法选择100例胃癌患者作为胃癌组，患者采用mFOLFOX6方案，2周为1个疗程，每位患者化疗4个疗程以上。另选择同期在医院体检的健康人群80例作为对照组，采用流式细胞仪技术检测两组外周血T淋巴细胞亚群水平。结果化疗前胃癌组外周血CD3+、CD4+、CD4+/CD8+显著低于对照组，CD8+显著高于对照组，差异有统计学意义（P﹤0.01）。Ⅰ～Ⅱ期胃癌患者外周血CD3+、CD4+、CD4+/CD8+显著高于Ⅲ～Ⅳ期，CD8+显著低于Ⅲ～Ⅳ期，差异有统计学意义（P﹤0.01）。不同疗效的胃癌患者化疗前外周血T淋巴细胞亚群水平比较差异无统计学意义（P﹥0.05），化疗后CR+PR组、SD组、PD组外周血CD3+、CD4+、CD4+/CD8+显著降低，CD8+显著上升，差异有统计学意义（P﹤0.01），且CR+PR组外周血CD3+、CD4+、CD4+/CD8+显著高于SD组和PD组，CD8+显著低于SD组和PD组，差异有统计学意义（P﹤0.01）。结论通过检测血清外周血T淋巴细胞亚群水平能够有助于评估胃癌的病情和预后。

Clinical significance of peripheral blood T lymphocyte subsets as prognostic markers in patients with gastric cancer

Objective To explore the clinical value of peripheral blood T lymphocyte subsets as markers for surveil-lance and prognosis in patients with gastric cancer (GC). Method A total of 100 cases of gastric cancer patients were en-rolled as GC group, treated with mFOLFOX6 regimen, with 2 weeks as 1 cycle, and each patient was given 4 cycles of chemotherapy at least. Another 80 cases of healthy adults in clinic during the same period were included as the control group, and the level of T lymphocyte subsets in peripheral blood of the two groups were detected by flow cytometry. Re-sult Before chemotherapy, the CD3+, CD4+, and CD4+/CD8+were significantly lower, and CD8+was higher in GC group compared with that of control group (P<0.05);The CD3+, CD4+, and CD4+/CD8+in GC patients of I-II stage were significant-ly higher than those of III-IV stage,while the CD8+in the former was significantly lower than that in the latter (P<0.01). There was no significant difference in T lymphocyte subsets level in peripheral blood of GC patients with different re-sponse before chemotherapy (P>0.05), After treatment, the CR+PR group, SD group, PD group had decreased peripheral blood CD3+, CD4+, and CD4+/CD8+, and increased CD8+(P<0.01), CD3+, CD4+, and CD4+/CD8+in CR+PR group were signifi-cantly higher than, while CD8+was significantly lower than those in SD group and PD group (P<0.01). Conclusion De-tecting the T lymphocyte subsets in peripheral blood is beneficial in monitoring disease condition and predicting progno-sis.