RECK基因在子痫前期患者胎盘的表达及其与MMP-2活化的关系

目的 通过检测RECK基因在子痫前期患者胎盘组织中的表达及其与MMP-2活化的关系,探讨RECK基因在胎盘滋养细胞浸润机制中的作用.方法 分别采用RT-PCR、Western blot检测正常妊娠孕妇(正常妊娠组,22例)、子痫前期孕妇(其中轻度22例,重度20例)胎盘组织中RECK mRNA和蛋白的表达;采用明胶酶谱法检测3组孕妇胎盘组织中MMP-2的活化比例.结果 轻度、重度子痫前期组胎盘组织中RECK mRNA、蛋白的表达水平均明显高于正常妊娠组,且重度子痫前期组RECK mRNA、蛋白的表达水平均明显高于轻度子痫前期组,组间比较差异均有统计学意义(均P<0.01);而轻度、重度子痫前期组MMP-2的活化比例均明显低于正常妊娠组,且重度子痫前期组MMP-2的活化比例明显低于轻度子痫前期组,组间比较差异均有统计学意义(均P<0.01).正常妊娠组、轻度子痫前期组、重度子痫前期组胎盘组织中RECK mRNA及其蛋白表达与MMP-2的活化比例均呈负相关关系(均P<0.01).结论 子痫前期孕妇胎盘组织中RECK表达异常升高,MMP-2活性受到抑制,可能参与了胎盘滋养细胞浅浸润过程.

Expression of RECK Gene in Placentas from Patients with Preeclampsia and Its Correlation with MMP-2 Activation

Objective To investigate the expression of RECK gene in placentas of patients with preeclampsia and its correlation with MMP-2 activation,and explore the possible roles of RECK gene in the placental trophoblast invasion mechanism.Methods RT-PCR and Western blot were used to detect the expression of RECK mRNA and protein respectively in the placental tissues of normal late pregnant women (normal pregnant group,22 cases) and pre-eclamptic patients(22 mild cases and 20 severe cases).Gelatinase zymography was used to determine MMP-2 activation ratio.Results The expression levels of RECK mRNA and protein from placenta tissues in mild,severe pre-eclamptic group were both significantly higher than those in nomal pregnant group.Moreover,the expression levels of RECK mRNA and protein in severe pre-eclamptic group were obviously increased as compared with those in mild pre-eclamptic group.There was significant difference among the three groups (all P<0.01).MMP-2 activation ratio in mild,severe pre-eclamptic group was significantly lower than that in normal pregnant group.MMP-2 activation ratio in severe pre-eclamptie groups was obviously reduced as compared with mild pre-eclamptic group.There was significant difference among the three groups(all P<0.01).The expression leVels of RECK mRNA and protein were significantly negatively correlated with MMP-2 activation ratio (both P<0.01).Conclusion The abnormal high expression of RECK and inhibition of MMP-2 activation in placentas of pre-eclamptic patients may participate in the process of placental trophoblast shallow invasion.