sICAM-1在重症肌无力患者血清及外周血单个核细胞培养上清液中的变化

目的：了解可溶性细胞间黏附分子-1（sICAM-1）在重症肌无力（MG）患者血清及外周血单个核细胞（PBMC）培养上清液中的变化情况。方法：用酶联免疫吸附法（ELISA）检测正常对照和MG患者，血清及PBMC培养上清液中sICAM-1的水平，并分析激素对上清液中sICAM-1的影响。结果：正常对照组与MG组血清sICAM-1的水平没有显著差异；而在PBMC培养上清液中，MG组高于正常对照组。加入地塞米松培养48h后，7例MG患者PBMC培养上清液中sICAM-1-1水平降低。结论：MG患者PBMC可分泌更多的sICAM-1，升高的sICAM-1可能是体内ICAM-1产生及循环过程的中间产物；ELISA检测MG患者PBMC培养上清液（加FBS）中sICAM-1的水平是一种有效的检测手段；此外，地塞米松可以下调PBMC分泌sICAM-1。

The Serum and Cultured-cell Supernatant Level of sICAM-1 in Myasthenia Gravis

Aim:To study the level of ICAM-1,especially soluble ICAM-1,in the serum and the supernatant of PBMC in patients with myasthenia gravis(MG).Methods:We use ELISA to quanlificate the level of sICAM-1 in the serum and in PBMC culture supernatant of patients with MG and the healthy controls.Results:There was no significant difference in the serum level of sICAM-1 between the patients with MG and the healthy controls.While the level of sICAM-1 in the supernatant of the patients with MG was higher than that in healthy control.The supernatant level of sICAM-1 of seven patients with MG co-cultured with dexametha- sone was decreased after 48 hours.Conclusion:PBMC secretes more sICAM-1 in MG patients than in normals.It is a better method to analyze the supernatant level of sICAM-1 in PBMC supernatant cultured with FBS in patients with MG.Dexamethasone can down-regulate the secretion of sICAM-1.