| MPTP诱导的小鼠帕金森病模型中相关脑区iNOS时程表达变化 |
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| 引用本文： | 董玉林,张志军,刘娟,郁晓燕,倪衡建. MPTP诱导的小鼠帕金森病模型中相关脑区iNOS时程表达变化[J]. 南通医学院学报, 2009, 29(1) |
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| 作者姓名： | 董玉林 张志军 刘娟 郁晓燕 倪衡建 |
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| 作者单位： | 南通大学医学院人体解剖学教研室,神经生物学研究所,江苏省神经再生重点实验室,南通226001 |
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| 基金项目： | 江苏省高校自然科学基金指导计划项目，南通大学医学院科研基金 |
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| 摘 要： | 目的:观察1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的小鼠急性损伤PD模型中纹状体(CPu)、黑质致密部(SNpc)、额叶皮层联合区(FrA)和腹侧被盖区(VTA)中iNOS基因和蛋白的时程表达变化。方法:用RT-PCR和Western blot方法对MPTP诱导的小鼠PD模型中相关脑区iNOS基因和蛋白的表达量进行检测。结果:MPTP诱导的小鼠PD模型中SNpc和CPu脑区iNOS基因和蛋白在给药后多个时间点均有显著性表达,其中1d是表达高峰;VTA和FrA脑区iNOS基因和蛋白的表达没有SNpc和CPu脑区表达显著。结论:表达增高的iNOS可能对SNpc和VTA脑区DA能神经元及CPu和FrA脑区的DA能神经元末梢起了损伤作用,从而导致了DA能神经元的减少及其末梢退变的发生。
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| 关 键 词： | 帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 诱导型一氧化氮合酶 小鼠 |
The interval diversification of iNOS expression in some brain regions of MPTP models of Parkinson's disease |
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| DONG Yulin,ZHANG Zhijun,LIU Juan,et al. The interval diversification of iNOS expression in some brain regions of MPTP models of Parkinson's disease[J]. ACTA Academiae Medicinae Nantong, 2009, 29(1) |
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| Authors: | DONG Yulin ZHANG Zhijun LIU Juan et al |
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| Affiliation: | Department of Human Anatomy;Medical School;Institute of Neurobiology;Nantong University;Jiangsu Province Key Laboratory of Neuroregeneration;Nantong 226001 |
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| Abstract: | Objective:To observe the time-course expression of iNOS mRNA and protein in CPu,SNpc,FrA and VTA of the MPTP model of PD. Methods: Semi-quantitative RT-PCR and Western blot methods were used to detect the expressions of iNOS mRNA and proteins in these four regions. Results: The expressions of iNOS mRNA and proteins in CPu and SNpc increased significantly at several time points in the MPTP model of PD,and a peak was observed on day1.The expressions of iNOS mRNA and proteins in VTA and FrA increased less than... |
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| Keywords: | Parkinson’s disease （PD） 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine iNOS Mouse |
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