| 大鼠局灶性脑缺血再灌注中iNOS来源的NO对细胞凋亡的影响 |
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| 引用本文： | 赵昱,马洪骏,张爱子,罗波,李陈莉,王慧娟.大鼠局灶性脑缺血再灌注中iNOS来源的NO对细胞凋亡的影响[J].河北医科大学学报,2005,26(4):241-244,253. |
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| 作者姓名： | 赵昱 马洪骏 张爱子 罗波 李陈莉 王慧娟 |
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| 作者单位： | 河北医科大学基础医学院组织胚胎学教研室,河北,石家庄,050017;石家庄市裕华区裕华西路卫生院,河北,石家庄,050017 |
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| 摘 要： | 目的 探讨大鼠局灶性脑缺血2h再灌注48h诱导型一氧化氮合酶(inducible nitric oxide synthase，iNOS)来源的一氧化氮(nitricoxide，NO)对神经元凋亡的影响。方法闭塞大鼠左侧大脑中动脉造成局灶性脑缺血模型，给予选择性iNOS抑制剂氨基胍，硝酸还原酶法检测NO含量。流式细胞术检测硝基酪氨酸表达，应用末端转移酶介导的缺口末端标记法(TdT-mediated dUTP nick ending labelling，TUNEL)及流式细胞术检测缺血2h再灌注48h细胞凋亡的变化。结果氨基胍可使缺血2h再灌注48h大鼠脑内TUNEL阳性细胞明显减少，细胞凋亡百分率降低，凋亡峰降低。结论iNOS来源的NO参与介导脑缺血再灌注晚期的细胞凋亡。
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| 关 键 词： | 脑缺血 脱噬作用 一氧化氮 大鼠 |
| 文章编号： | 1007-3205(2005)04-0241-04 |
EFFECTS OF iNOS-DERIVED NO ON APOPTOSIS FOLLOWING FOCAL CEREBRAL ISCHEMIA AND REPERFUSION IN RATS |
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| ZHAO Yu,MA Hong-jun,ZHANG Ai-zi,Luo Bo,LI Chen-li,WANG Hui-juan.EFFECTS OF iNOS-DERIVED NO ON APOPTOSIS FOLLOWING FOCAL CEREBRAL ISCHEMIA AND REPERFUSION IN RATS[J].Journal of Hebei Medical University,2005,26(4):241-244,253. |
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| Authors: | ZHAO Yu MA Hong-jun ZHANG Ai-zi Luo Bo LI Chen-li WANG Hui-juan |
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| Institution: | ZHAO Yu~1,MA Hong-jun~1,ZHANG Ai-zi~1,LUO Bo~2,LI Chen-li~1,WANG Hui-juan~1 |
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| Abstract: | ObjectiveTo investigate the effects of iNOS-derived NO on apoptosis following focal cerebral ischemia and reperfusion in rats. MethodsFocal cerebral ischemic model was induced by the occlusion of left middle cerebral artery. Aminoguandine, a selective inhibitor of iNOS, was given intraperitoneally. The rats were sacrificed at 48 h of reperfusion after 2 h of ischemia. The content of NO was measured by nitriate reductase method.The expression of NT was examined by flow cytometry.The cellular apoptosis was examined by flow cytometry and TUNEL. ResultsThe number of TUNEL positive cells and the apoptotic percentage were decreased markedly in Aminoguandine groups. ConclusioniNOS-derived NO may play a role in apoptosis following focal cerebral ischemia and reperfusion. |
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| Keywords: | cerebral ischemia apoptosis nitric oxide rats |
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