首页 | 本学科首页   官方微博 | 高级检索  
检索        


Urinary N-acetyl-β-D-glucosaminidase activity in rabbits with experimental hypercalciuria
Authors:Mehmet Türkmen  Salih Kavukcu  Hüray Islekel  Sülen Sario?lu  Hülya Akhunlar  Neriman Gökden  Ataman Güre
Institution:(1) Department of Pediatrics, Dokuz Eylül University, Faculty of Medicine, Inciraltı-Izmir, Turkey, TR;(2) Department of Biochemistry, Dokuz Eylül University, Faculty of Medicine, Inciraltı-Izmir, Turkey, TR;(3) Department of Pathology, Dokuz Eylül University, Faculty of Medicine, Inciraltı-Izmir, Turkey, TR;(4) Department of Physiology, Dokuz Eylül University, Faculty of Medicine, Inciraltı-Izmir, Turkey, TR
Abstract:Routinely used renal function tests remain normal in uncomplicated hypercalciuria. The aim of this study was to assess the value of N-acetyl-β-D-glucosaminidase (NAG), a sensitive marker of renal proximal tubular damage, in experimental hypercalciuria. Oral calcium providing 75 mg/kg per day elementary calcium and 20,000 IU/day vitamin D3 was administered for 15 days to 7 rabbits (Orytolagus cuniculus-New Zealand white) and 7 rabbits were given placebo as a control group. Serum calcium, phosphorus, and alkaline phosphatase, daily urinary calcium excretion and NAG/creatinine ratio were measured before and after drug administration. Kidneys were examined macroscopically and microscopically following the study period. Serum calcium, phosphorus and urinary calcium excretion increased, while alkaline phosphatase decreased significantly in response to drug treatment 10.8±1.5 vs. 12.2±1.3 mg/dl, 4.6±0.6 vs. 6.7±0.7 mg/dl, 22.3±8.3 vs. 46.8±22.5 mg/kg per day, and 138.0±57.1 vs. 70.1±33.1 IU/l, respectively (P <0.05)]. The NAG/creatinine ratio prior to the study (0.5±0.1 mU/mg) was significantly different from that after the study (5.4±1.5 mU/mg, P <0.01). In the control group, changes in serum and urinary parameters were not significant (P >0.05). The relationship between the urinary NAG/creatinine ratio and the daily urinary calcium excretion was statistically significant (r = 0.67, P <0.05). In the study group, nephrocalcinosis was present in all rabbits except 1 (85.7%), whereas none of the control group rabbits had nephrocalcinosis. In conclusion, in rabbits urinary NAG excretion increases significantly in nephrocalcinosis induced by hypercalciuria. Received February 12, 1996; received in revised form November 25, 1996; accepted November 27, 1996
Keywords:: Hypercalciuria  Nephrocalcinosis  Rabbit  N-Acetyl-β  -D glucosaminidase
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号