高效液相色谱法检测米雅利桑爱儿A颗粒中B族维生素

 目的:测定米稚利桑爱儿A颗粒中维生素B₆、泛酸钙、维生素B_{2的含量。方法:米用高效液相色谱法，用Waters CLC-ODS柱，以乙睛-0. 25 mol· L^-1磷酸二氢钾(pH 3.2±0.4)为流动相梯度洗脱，对乙酰氨基酚为内标，泛酸钙检测波长为210 nm，维生素B6、内标和维生素B2的检测波长为280 nm。结果:维生素B6}、泛酸钙、维生素B₂ 分别在20～130μg·ml^-1( r = 0.999 3,n=6),20～130μg·ml^-1(r =0.998 6,n=6), 5～30μg · ml^-1(r = 0.999 3, n=6)浓度范围内呈线性关系。维生素B6、泛酸钙、维生素B₂平均回收率分别为99.7%,RSD=0.86%;100.0%,RSD=0.95% ;98.2%, RSD=1.02%。结论:方法简便、快速、准确、重现性好。

Determination of vitamin B 6,calcium pantothenate and vitamin B 2 in Miyarisan Aiji A fine granules by HPLC

OBJECTIVE: To make an assay of vitamin B₆, calcium pantothenate and vitamin B₂ in Miyarisan Aiji A fine granules. METHOD: A HPLC method was chromatographed on Waters CLC-ODS column with a mobile phase of acetonitrile-0.25 mol·L^-1potassium dihydrogen phosphose(pH3.2± 0.4)(varying from 8 :92 to 25:75 in 25 minutes) , acetaminophen as internal substance. UV detections were performod at 210 nm for calcium pantothenate and 280 nm for vitaminB₆，acetaminophen and vitaminB₂.RESULTS: The standard curves for vitaminB₆，calcium pantothenate and vitamin B₂ were linear in the range of 20～130μg·ml^-1(r=0.999 3,n＝6)，20～130μg·ml^-1(r=0.998 6,n=6),5～30μg·ml^-1(r=0.999 3,n=6) respectively. The average recoveries of vitamin B₆, calcium pantothenate and vitamin B₂at different concentration were 99.7%，，100.0% and 98.2% respectively, their RSD was 0.86%，0.95%，1.02%(n=5).CONCLUSION: The method is convenient, rapid, accurate and precise.