| 端粒酶反义核酸对乳腺癌细胞生长的抑制作用 |
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| 引用本文： | 赵丽,曹英林,孙汶生.端粒酶反义核酸对乳腺癌细胞生长的抑制作用[J].基础医学与临床,2002,22(2):147-149. |
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| 作者姓名： | 赵丽 曹英林 孙汶生 |
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| 作者单位： | 山东大学,医学院,免疫学研究所,济南,250012 |
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| 基金项目： | 山东卫生厅基金 (9810 10 10 3) |
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| 摘 要： | 为了探讨针对人类端粒酶RNA（hTR）基因的反义寡核苷酸（AS－ODN）对乳腺癌细胞系MCF－7的影响，将AS－OND作用于细胞。进行细胞计数，MTT比色法测细胞生长抑制率，PCR－ELISA法测端粒酶活性，流式细胞仪测细胞周期与凋亡。结果表明该AS－ODN能抑制MCF－7细胞生长，降低端粒酶活性并诱导细胞凋亡。针对hTR的AS－ODN对治疗恶性肿瘤有潜在意义。
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| 关 键 词： | 乳腺癌 细胞生长 端粒酶 反义核酸 乳腺肿瘤 抑癌作用 |
| 文章编号： | 1001-6325(2002)02-0147-03 |
| 修稿时间： | 2001年8月30日 |
Inhibitory effects of telomerase antisense oligonucleotides on growth of breast cancer cells |
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| ZHAO Li,CAO Ying-lin,SUN Wen-sheng.Inhibitory effects of telomerase antisense oligonucleotides on growth of breast cancer cells[J].Basic Medical Sciences and Clinics,2002,22(2):147-149. |
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| Authors: | ZHAO Li CAO Ying-lin SUN Wen-sheng |
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| Abstract: | To investigate the effects of antisense oligonucleotides (AS-ODN) against human telomerase RNA (hTR) on breast cancer cell line MCF-7. Cells were incubated with the AS-ODN and then the number of the cells were counted. The inhibition rates were measured by MTT. Telomerase activity was detected by PCR-ELISA and apoptosis by was examined flow cytometry. Inhibition of cell growth telomerase activity and induction of apoptosis were found. The AS-ODN against hTR has potential effects on malignant cancer. |
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| Keywords: | telomerase antisense oligonucleotides breast cancer |
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