当归红芪超滤物对心肌细胞Hsp70表达的影响

目的：探讨当归红芪超滤物对凋亡心肌细胞热休克蛋白70（Hsp70）表达的影响，阐明当归红芪超滤物对心肌细胞的抗氧化作用。方法：原代培养的Wistar乳鼠心肌细胞用高浓度的H₂O₂（400 μmol?L^-1）建立细胞凋亡模型，用不同浓度（3.75、7.50和15.00　g?L^-1）的当归红芪超滤物进行干预，倒置显微镜下观察各实验组心肌细胞搏动频率，流式细胞仪检测心肌细胞凋亡率，分别用RT-PCR技术和Western blotting技术检测心肌细胞中Hsp70基因及蛋白的表达情况。结果：与对照组比较，H2O2损伤组心肌细胞搏动频率显著降低(P<0.01)，细胞凋亡率显著增加(P<0.01)，Hsp70 mRNA及蛋白表达量升高 (P<0.05，P<0.01)；与H₂O₂损伤组比较，当归红芪超滤物高、中、低剂量组心肌细胞搏动频率显著增加，但低于对照组(P<0.01)，凋亡率显著降低(P<0.01)，Hsp70 mRNA及蛋白表达量显著升高（P<0.05，P<0.01）。结论：当归红芪超滤物具有抗氧化损伤心肌细胞凋亡的作用，且可上调心肌细胞Hsp70的表达。

Effect of ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys on expression of Hsp70 in cardiomyocytes

Objective To investigate the effect of the ultra-filtration extract mixture from Angelica Sinensis and Hedysarum Polybotrys on heat shock protein 70 (Hsp70) expression in cardiomyocytes, and clarify its antioxidation on cardiomyocytes. Methods Myocardial ceils from 1 - 3 d neonatal rats were cultivated in DF medium and the cellular injury was induced by H_2O_2 (400μmol · L~(-1)).The ultra-filtration extract mixtures were given in three doses of 3.75, 7.50, and 15.00 g · L~(-1).The heating rates of cardiomyocytes were observed by phase-contrast microscope. The apoptotic rates of cardiomyocytes were measured by flow cytometry. The expressions of Hsp70 mRNA and protein in cardiomyocytes were measured by RT-PCR and Western blotting.Results Compared with normal control group, the beating rates of cardiomyocytes were significantly decreased (P<0.01) and the apoptotie rates of cardiomyocytes were significantly increased (P<0.01) in model group, and the expressions of Hsp70 mRNA and protein in cardiomyocytes were increased (P<0.05, P<0.01).Compared with model group, the beating rates of ca rdiomyocytes were significantly increased (P<0.01) and the apoptotic rates were significantly decreased (P<0.01) in each treatment group, and the expressions of HspT0 mRNA and protein in cardiomyocytes were significantly increased (P < 0.01, P < 0.05).Conclusion The ultra-filtration extract mixture has the protective effects on cardiomyocytes injured by H_2O_2 and can up-regulate the Hsp70 expression in cardiomyocytes.